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| | ==Cell attachment protein VP8* of a human rotavirus specifically interacts with A-type histo-blood group antigen== | | ==Cell attachment protein VP8* of a human rotavirus specifically interacts with A-type histo-blood group antigen== |
| - | <StructureSection load='4drr' size='340' side='right' caption='[[4drr]], [[Resolution|resolution]] 1.50Å' scene=''> | + | <StructureSection load='4drr' size='340' side='right'caption='[[4drr]], [[Resolution|resolution]] 1.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4drr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rotavirus_sp. Rotavirus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DRR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DRR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4drr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rotavirus_sp. Rotavirus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DRR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DRR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4drv|4drv]], [[4ds0|4ds0]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4drr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4drr OCA], [https://pdbe.org/4drr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4drr RCSB], [https://www.ebi.ac.uk/pdbsum/4drr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4drr ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10970 Rotavirus sp.])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4drr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4drr OCA], [http://pdbe.org/4drr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4drr RCSB], [http://www.ebi.ac.uk/pdbsum/4drr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4drr ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/Q86169_9REOV Q86169_9REOV]] Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment.[SAAS:SAAS00136874] VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact.[SAAS:SAAS00136880] | + | [[https://www.uniprot.org/uniprot/Q86169_9REOV Q86169_9REOV]] Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment.[SAAS:SAAS00136874] VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact.[SAAS:SAAS00136880] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[VP4|VP4]]
| + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| - | *[[Virus coat protein|Virus coat protein]] | + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Rotavirus sp]] | | [[Category: Rotavirus sp]] |
| - | [[Category: Cortes-Penfield, N W]] | + | [[Category: Cortes-Penfield NW]] |
| - | [[Category: Crawford, S E]] | + | [[Category: Crawford SE]] |
| - | [[Category: Czako, R]] | + | [[Category: Czako R]] |
| - | [[Category: Estes, M K]] | + | [[Category: Estes MK]] |
| - | [[Category: Hu, L]] | + | [[Category: Hu L]] |
| - | [[Category: Pendu, J Le]] | + | [[Category: Le Pendu J]] |
| - | [[Category: Prasad, B V.V]] | + | [[Category: Prasad BVV]] |
| - | [[Category: Smith, D F]] | + | [[Category: Smith DF]] |
| - | [[Category: Cell attachment factor]]
| + | |
| - | [[Category: Galectin-fold]]
| + | |
| - | [[Category: Histo blood group antigen]]
| + | |
| - | [[Category: Otavirus]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
[Q86169_9REOV] Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment.[SAAS:SAAS00136874] VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact.[SAAS:SAAS00136880]
Publication Abstract from PubMed
As with many other viruses, the initial cell attachment of rotaviruses, which are the major causative agent of infantile gastroenteritis, is mediated by interactions with specific cellular glycans. The distally located VP8* domain of the rotavirus spike protein VP4 (ref. 5) mediates such interactions. The existing paradigm is that 'sialidase-sensitive' animal rotavirus strains bind to glycans with terminal sialic acid (Sia), whereas 'sialidase-insensitive' human rotavirus strains bind to glycans with internal Sia such as GM1 (ref. 3). Although the involvement of Sia in the animal strains is firmly supported by crystallographic studies, it is not yet known how VP8* of human rotaviruses interacts with Sia and whether their cell attachment necessarily involves sialoglycans. Here we show that VP8* of a human rotavirus strain specifically recognizes A-type histo-blood group antigen (HBGA) using a glycan array screen comprised of 511 glycans, and that virus infectivity in HT-29 cells is abrogated by anti-A-type antibodies as well as significantly enhanced in Chinese hamster ovary cells genetically modified to express the A-type HBGA, providing a novel paradigm for initial cell attachment of human rotavirus. HBGAs are genetically determined glycoconjugates present in mucosal secretions, epithelia and on red blood cells, and are recognized as susceptibility and cell attachment factors for gastric pathogens like Helicobacter pylori and noroviruses. Our crystallographic studies show that the A-type HBGA binds to the human rotavirus VP8* at the same location as the Sia in the VP8* of animal rotavirus, and suggest how subtle changes within the same structural framework allow for such receptor switching. These results raise the possibility that host susceptibility to specific human rotavirus strains and pathogenesis are influenced by genetically controlled expression of different HBGAs among the world's population.
Cell attachment protein VP8* of a human rotavirus specifically interacts with A-type histo-blood group antigen.,Hu L, Crawford SE, Czako R, Cortes-Penfield NW, Smith DF, Le Pendu J, Estes MK, Prasad BV Nature. 2012 Apr 15;485(7397):256-9. doi: 10.1038/nature10996. PMID:22504179[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hu L, Crawford SE, Czako R, Cortes-Penfield NW, Smith DF, Le Pendu J, Estes MK, Prasad BV. Cell attachment protein VP8* of a human rotavirus specifically interacts with A-type histo-blood group antigen. Nature. 2012 Apr 15;485(7397):256-9. doi: 10.1038/nature10996. PMID:22504179 doi:10.1038/nature10996
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