4dt1

From Proteopedia

(Difference between revisions)

Revision as of 08:37, 21 September 2022

Crystal structure of the Psy3-Csm2 complex

Structural highlights

4dt1 is a 2 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CSM2_YEAST] Involved in chromosome segregation during meiosis. Promotes efficient recombinational repair and functions in the protection of the genome from spontaneous and induced DNA damage like mutations and gross chromosomal rearrangements (GCRs).^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

The yeast Shu complex, consisting of the proteins Shu1, Shu2, Psy3, and Csm2, maintains genomic stability by coupling post-replication repair to homologous recombination. However, a lack of biochemical and structural information on the Shu proteins precludes revealing their precise roles within the pathway. Here, we report on the 1.9-A crystal structure of the Psy3-Csm2 complex. The crystal structure shows that Psy3 forms a heterodimer with Csm2 mainly through a hydrophobic core. Unexpectedly, Psy3 and Csm2 share a similar architecture that closely resembles the ATPase core domain of Rad51. The L2 loop present in Psy3 and Csm2 is similar to that of Rad51 and confers the DNA binding activity of the Shu complex. As with Rad51, the Shu complex appears to form a nucleoprotein filament by binding nonspecifically to DNA. Structure-based mutagenesis studies have demonstrated that the DNA binding activity of the Shu complex is essential for repair of the methyl methanesulfonate-induced DNA damage. Our findings provide good foundations for the understanding of the Srs2 regulation by the Shu complex.

Structural analysis of Shu proteins reveals a DNA binding role essential for resisting damage.,Tao Y, Li X, Liu Y, Ruan J, Qi S, Niu L, Teng M J Biol Chem. 2012 Jun 8;287(24):20231-9. doi: 10.1074/jbc.M111.334698. Epub 2012 , Mar 30. PMID:22465956^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rabitsch KP, Toth A, Galova M, Schleiffer A, Schaffner G, Aigner E, Rupp C, Penkner AM, Moreno-Borchart AC, Primig M, Esposito RE, Klein F, Knop M, Nasmyth K. A screen for genes required for meiosis and spore formation based on whole-genome expression. Curr Biol. 2001 Jul 10;11(13):1001-9. PMID:11470404
↑ Huang ME, Rio AG, Nicolas A, Kolodner RD. A genomewide screen in Saccharomyces cerevisiae for genes that suppress the accumulation of mutations. Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11529-34. Epub 2003 Sep 12. PMID:12972632 doi:http://dx.doi.org/10.1073/pnas.2035018100
↑ Smith S, Hwang JY, Banerjee S, Majeed A, Gupta A, Myung K. Mutator genes for suppression of gross chromosomal rearrangements identified by a genome-wide screening in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):9039-44. Epub 2004 Jun 7. PMID:15184655 doi:10.1073/pnas.0403093101
↑ Shor E, Weinstein J, Rothstein R. A genetic screen for top3 suppressors in Saccharomyces cerevisiae identifies SHU1, SHU2, PSY3 and CSM2: four genes involved in error-free DNA repair. Genetics. 2005 Mar;169(3):1275-89. Epub 2005 Jan 16. PMID:15654096 doi:10.1534/genetics.104.036764
↑ Ball LG, Zhang K, Cobb JA, Boone C, Xiao W. The yeast Shu complex couples error-free post-replication repair to homologous recombination. Mol Microbiol. 2009 Jul;73(1):89-102. Epub 2009 Jun 1. PMID:19496932 doi:MMI6748
↑ Tao Y, Li X, Liu Y, Ruan J, Qi S, Niu L, Teng M. Structural analysis of Shu proteins reveals a DNA binding role essential for resisting damage. J Biol Chem. 2012 Jun 8;287(24):20231-9. doi: 10.1074/jbc.M111.334698. Epub 2012 , Mar 30. PMID:22465956 doi:10.1074/jbc.M111.334698

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4dt1"

Categories: Large Structures | Saccharomyces cerevisiae S288C | Niu L | Tao Y | Teng M

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Psy3-Csm2 complex==
-<StructureSection load='4dt1' size='340' side='right' caption='[[4dt1]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='4dt1' size='340' side='right'caption='[[4dt1]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4dt1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DT1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DT1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4dt1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DT1 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CSM2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), PSY3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dt1 OCA], [https://pdbe.org/4dt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dt1 RCSB], [https://www.ebi.ac.uk/pdbsum/4dt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dt1 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dt1 OCA], [http://pdbe.org/4dt1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dt1 RCSB], [http://www.ebi.ac.uk/pdbsum/4dt1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dt1 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CSM2_YEAST CSM2_YEAST]] Involved in chromosome segregation during meiosis. Promotes efficient recombinational repair and functions in the protection of the genome from spontaneous and induced DNA damage like mutations and gross chromosomal rearrangements (GCRs).<ref>PMID:11470404</ref> <ref>PMID:12972632</ref> <ref>PMID:15184655</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref>  [[http://www.uniprot.org/uniprot/PSY3_YEAST PSY3_YEAST]] Required for resistance to the DNA-damaging agents methyl methanesulfonate (MMS), cisplatin and oxaliplatin, but not to mitomycin C. Plays a role in protection against mutation accumulation. May be a component of the recombination-repair pathway.<ref>PMID:12149442</ref> <ref>PMID:12482937</ref> <ref>PMID:12972632</ref> <ref>PMID:15173006</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref>
+[[https://www.uniprot.org/uniprot/CSM2_YEAST CSM2_YEAST]] Involved in chromosome segregation during meiosis. Promotes efficient recombinational repair and functions in the protection of the genome from spontaneous and induced DNA damage like mutations and gross chromosomal rearrangements (GCRs).<ref>PMID:11470404</ref> <ref>PMID:12972632</ref> <ref>PMID:15184655</ref> <ref>PMID:15654096</ref> <ref>PMID:19496932</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Baker's yeast]]
+[[Category: Large Structures]]
-[[Category: Niu, L]]
+[[Category: Saccharomyces cerevisiae S288C]]
-[[Category: Tao, Y]]
+[[Category: Niu L]]
-[[Category: Teng, M]]
+[[Category: Tao Y]]
-[[Category: Dna binding protein]]
+[[Category: Teng M]]
-[[Category: Dna damage repair]]
-[[Category: Protein complex]]
-[[Category: Reca-like fold]]

4dt1

From Proteopedia

Revision as of 08:37, 21 September 2022

Crystal structure of the Psy3-Csm2 complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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