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| | ==Crystal structure of yeast Puf4p RNA binding domain in complex with HO-4BE mutant RNA== | | ==Crystal structure of yeast Puf4p RNA binding domain in complex with HO-4BE mutant RNA== |
| - | <StructureSection load='4dzs' size='340' side='right' caption='[[4dzs]], [[Resolution|resolution]] 3.14Å' scene=''> | + | <StructureSection load='4dzs' size='340' side='right'caption='[[4dzs]], [[Resolution|resolution]] 3.14Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4dzs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DZS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DZS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4dzs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DZS FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bx2|3bx2]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dzs OCA], [https://pdbe.org/4dzs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dzs RCSB], [https://www.ebi.ac.uk/pdbsum/4dzs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dzs ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PUF4, YGL014W, YGL023 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dzs OCA], [http://pdbe.org/4dzs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dzs RCSB], [http://www.ebi.ac.uk/pdbsum/4dzs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dzs ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PUF4_YEAST PUF4_YEAST]] Is not essential for haploid growth, but may affect diploid formation. | + | [[https://www.uniprot.org/uniprot/PUF4_YEAST PUF4_YEAST]] Is not essential for haploid growth, but may affect diploid formation. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | + | [[Category: Large Structures]] |
| - | [[Category: Hall, T M.T]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Qiu, C]] | + | [[Category: Hall TMT]] |
| - | [[Category: Puf rna binding domain]] | + | [[Category: Qiu C]] |
| - | [[Category: Rna]]
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| - | [[Category: Rna binding]]
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| - | [[Category: Rna binding protein-rna complex]]
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| Structural highlights
Function
[PUF4_YEAST] Is not essential for haploid growth, but may affect diploid formation.
Publication Abstract from PubMed
mRNA control hinges on the specificity and affinity of proteins for their RNA binding sites. Regulatory proteins must bind their own sites and reject even closely related noncognate sites. In the PUF [Pumilio and fem-3 binding factor (FBF)] family of RNA binding proteins, individual proteins discriminate differences in the length and sequence of binding sites, allowing each PUF to bind a distinct battery of mRNAs. Here, we show that despite these differences, the pattern of RNA interactions is conserved among PUF proteins: the two ends of the PUF protein make critical contacts with the two ends of the RNA sites. Despite this conserved "two-handed" pattern of recognition, the RNA sequence is flexible. Among the binding sites of yeast Puf4p, RNA sequence dictates the pattern in which RNA bases are flipped away from the binding surface of the protein. Small differences in RNA sequence allow new modes of control, recruiting Puf5p in addition to Puf4p to a single site. This embedded information adds a new layer of biological meaning to the connections between RNA targets and PUF proteins.
Patterns and plasticity in RNA-protein interactions enable recruitment of multiple proteins through a single site.,Valley CT, Porter DF, Qiu C, Campbell ZT, Hall TM, Wickens M Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6054-9. Epub 2012 Mar 30. PMID:22467831[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Valley CT, Porter DF, Qiu C, Campbell ZT, Hall TM, Wickens M. Patterns and plasticity in RNA-protein interactions enable recruitment of multiple proteins through a single site. Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6054-9. Epub 2012 Mar 30. PMID:22467831 doi:10.1073/pnas.1200521109
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