1ihx

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[[Image:1ihx.jpg|left|200px]]
[[Image:1ihx.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1ihx |SIZE=350|CAPTION= <scene name='initialview01'>1ihx</scene>, resolution 2.80&Aring;
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The line below this paragraph, containing "STRUCTURE_1ihx", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=SND:THIONICOTINAMIDE-ADENINE-DINUCLEOTIDE'>SND</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1ihx| PDB=1ihx | SCENE= }}
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|RELATEDENTRY=[[1ihy|1IHY]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ihx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ihx OCA], [http://www.ebi.ac.uk/pdbsum/1ihx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ihx RCSB]</span>
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}}
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'''Crystal structure of two D-glyceraldehyde-3-phosphate dehydrogenase complexes: a case of asymmetry'''
'''Crystal structure of two D-glyceraldehyde-3-phosphate dehydrogenase complexes: a case of asymmetry'''
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==Reference==
==Reference==
Structures of D-glyceraldehyde-3-phosphate dehydrogenase complexed with coenzyme analogues., Shen YQ, Song SY, Lin ZJ, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1287-97. Epub 2002, Jul 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12136140 12136140]
Structures of D-glyceraldehyde-3-phosphate dehydrogenase complexed with coenzyme analogues., Shen YQ, Song SY, Lin ZJ, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1287-97. Epub 2002, Jul 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12136140 12136140]
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[[Category: Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)]]
 
[[Category: Palinurus versicolor]]
[[Category: Palinurus versicolor]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Shen, Y Q.]]
[[Category: Shen, Y Q.]]
[[Category: Song, S Y.]]
[[Category: Song, S Y.]]
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[[Category: asymmetry]]
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[[Category: Asymmetry]]
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[[Category: gapdh]]
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[[Category: Gapdh]]
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[[Category: snad]]
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[[Category: Snad]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:01:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:20:03 2008''
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Revision as of 17:01, 2 May 2008

Image:1ihx.jpg

Template:STRUCTURE 1ihx

Crystal structure of two D-glyceraldehyde-3-phosphate dehydrogenase complexes: a case of asymmetry


Overview

Crystal structures of GAPDH from Palinurus versicolor complexed with two coenzyme analogues, SNAD(+) and ADP-ribose, were determined by molecular replacement and refined at medium resolution to acceptable crystallographic factors and reasonable stereochemistry. ADP-ribose in the ADP-ribose-GAPDH complex adopts a rather extended conformation. The interactions between ADP-ribose and GAPDH are extensive and in a fashion dissimilar to the coenzyme NAD(+). This accounts for the strong inhibiting ability of ADP-ribose. The conformational changes induced by ADP-ribose binding are quite different to those induced by NAD(+) binding. This presumably explains the non-cooperative behaviour of the ADP-ribose binding. Unexpectedly, the SNAD(+)-GAPDH complex reveals pairwise asymmetry. The asymmetry is significant, including the SNAD(+) molecule, active-site structure and domain motion induced by the coenzyme analogue. In the yellow or red subunits [nomenclature of subunits is as in Buehner et al. (1974). J. Mol. Biol. 90, 25-49], SNAD(+) binds similarly, as does NAD(+) in holo-GAPDH. While, in the green or blue subunit, the SNAD(+) binds in a non-productive manner, resulting in a disordered thionicotinamide ring and rearranged active-site residues. The conformation seen in the yellow and red subunits of SNAD(+)-GAPDH is likely to represent the functional state of the enzyme complex in solution and thus accounts for the substrate activity of SNAD(+). A novel type of domain motion is observed for the binding of the coenzyme analogues to GAPDH. The possible conformational transitions involved in the coenzyme binding and the important role of the nicotinamide group are discussed.

About this Structure

1IHX is a Single protein structure of sequence from Palinurus versicolor. Full crystallographic information is available from OCA.

Reference

Structures of D-glyceraldehyde-3-phosphate dehydrogenase complexed with coenzyme analogues., Shen YQ, Song SY, Lin ZJ, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1287-97. Epub 2002, Jul 20. PMID:12136140 Page seeded by OCA on Fri May 2 20:01:18 2008

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