1ii7
From Proteopedia
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[[Image:1ii7.gif|left|200px]] | [[Image:1ii7.gif|left|200px]] | ||
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'''Crystal structure of P. furiosus Mre11 with manganese and dAMP''' | '''Crystal structure of P. furiosus Mre11 with manganese and dAMP''' | ||
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[[Category: Tainer, J A.]] | [[Category: Tainer, J A.]] | ||
[[Category: Woo, T T.]] | [[Category: Woo, T T.]] | ||
| - | [[Category: | + | [[Category: Damp]] |
| - | [[Category: | + | [[Category: Dna double-strand break repair]] |
| - | [[Category: | + | [[Category: Manganese]] |
| - | [[Category: | + | [[Category: Mre11]] |
| - | [[Category: | + | [[Category: Rad50]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:01:53 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:01, 2 May 2008
Crystal structure of P. furiosus Mre11 with manganese and dAMP
Overview
To clarify functions of the Mre11/Rad50 (MR) complex in DNA double-strand break repair, we report Pyrococcus furiosus Mre11 crystal structures, revealing a protein phosphatase-like, dimanganese binding domain capped by a unique domain controlling active site access. These structures unify Mre11's multiple nuclease activities in a single endo/exonuclease mechanism and reveal eukaryotic macromolecular interaction sites by mapping human and yeast Mre11 mutations. Furthermore, the structure of the P. furiosus Rad50 ABC-ATPase with its adjacent coiled-coil defines a compact Mre11/Rad50-ATPase complex and suggests that Rad50-ATP-driven conformational switching directly controls the Mre11 exonuclease. Electron microscopy, small angle X-ray scattering, and ultracentrifugation data of human and P. furiosus MR reveal a dual functional complex consisting of a (Mre11)2/(Rad50)2 heterotetrameric DNA processing head and a double coiled-coil linker.
About this Structure
1II7 is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.
Reference
Structural biochemistry and interaction architecture of the DNA double-strand break repair Mre11 nuclease and Rad50-ATPase., Hopfner KP, Karcher A, Craig L, Woo TT, Carney JP, Tainer JA, Cell. 2001 May 18;105(4):473-85. PMID:11371344 Page seeded by OCA on Fri May 2 20:01:53 2008
