7xe0
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of plant NLR Sr35 resistosome== | |
| + | <StructureSection load='7xe0' size='340' side='right'caption='[[7xe0]], [[Resolution|resolution]] 3.33Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7xe0]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Puccinia_graminis_f._sp._tritici Puccinia graminis f. sp. tritici] and [https://en.wikipedia.org/wiki/Triticum_monococcum Triticum monococcum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XE0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XE0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xe0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xe0 OCA], [https://pdbe.org/7xe0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xe0 RCSB], [https://www.ebi.ac.uk/pdbsum/7xe0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xe0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/S5ABD6_TRIMO S5ABD6_TRIMO]] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. The direct recognition mechanism of pathogen effectors by coiled-coil NLRs (CNLs) remains unclear. We demonstrate that the Triticum monococcum CNL Sr35 directly recognizes the pathogen effector AvrSr35 from Puccinia graminis f. sp. tritici and report a cryo-electron microscopy structure of Sr35 resistosome and a crystal structure of AvrSr35. We show that AvrSr35 forms homodimers that are disassociated into monomers upon direct recognition by the leucine-rich repeat domain of Sr35, which induces Sr35 resistosome assembly and the subsequent immune response. The first 20 amino-terminal residues of Sr35 are indispensable for immune signaling but not for plasma membrane association. Our findings reveal the direct recognition and activation mechanism of a plant CNL and provide insights into biochemical function of Sr35 resistosome. | ||
| - | + | Pathogen effector AvrSr35 triggers Sr35 resistosome assembly via a direct recognition mechanism.,Zhao YB, Liu MX, Chen TT, Ma X, Li ZK, Zheng Z, Zheng SR, Chen L, Li YZ, Tang LR, Chen Q, Wang P, Ouyang S Sci Adv. 2022 Sep 9;8(36):eabq5108. doi: 10.1126/sciadv.abq5108. Epub 2022 Sep 9. PMID:36083908<ref>PMID:36083908</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7xe0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Puccinia graminis f. sp. tritici]] | ||
| + | [[Category: Triticum monococcum]] | ||
| + | [[Category: Li ZK]] | ||
| + | [[Category: Liu MX]] | ||
| + | [[Category: Ouyang SY]] | ||
| + | [[Category: Zhao YB]] | ||
Revision as of 06:19, 28 September 2022
Cryo-EM structure of plant NLR Sr35 resistosome
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