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1ij1
From Proteopedia
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[[Image:1ij1.jpg|left|200px]] | [[Image:1ij1.jpg|left|200px]] | ||
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| - | | | + | {{STRUCTURE_1ij1| PDB=1ij1 | SCENE= }} |
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'''GCN4-pVLT Coiled-coil Trimer with Threonine at the d(12) Position''' | '''GCN4-pVLT Coiled-coil Trimer with Threonine at the d(12) Position''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1IJ1 is a [[Single protein]] structure | + | 1IJ1 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Kim, P S.]] | [[Category: Kim, P S.]] | ||
[[Category: Malashkevich, V N.]] | [[Category: Malashkevich, V N.]] | ||
| - | [[Category: | + | [[Category: Coiled coil]] |
| - | [[Category: | + | [[Category: Gcn4]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:03:20 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:03, 2 May 2008
GCN4-pVLT Coiled-coil Trimer with Threonine at the d(12) Position
Overview
Coiled coils, estimated to constitute 3-5% of the encoded residues in most genomes, are characterized by a heptad repeat, (abcdefg)(n), where the buried a and d positions form the interface between multiple alpha-helices. Although generally hydrophobic, a substantial fraction ( approximately 20%) of these a- and d-position residues are polar or charged. We constructed variants of the well-characterized coiled coil GCN4-p1 with a single polar residue (Asn, Gln, Ser, or Thr) at either an a or a d position. The stability and oligomeric specificity of each variant were measured, and crystal structures of coiled-coil trimers with threonine or serine at either an a or a d position were determined. The structures show how single polar residues in the interface affect not only local packing, but also overall coiled-coil geometry as seen by changes in the Crick supercoil parameters and core cavity volumes.
About this Structure
1IJ1 is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Buried polar residues in coiled-coil interfaces., Akey DL, Malashkevich VN, Kim PS, Biochemistry. 2001 May 29;40(21):6352-60. PMID:11371197 Page seeded by OCA on Fri May 2 20:03:20 2008
