8cxp
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Characterisation of a Seneca Valley Virus Thermostable Mutant== | |
| + | <StructureSection load='8cxp' size='340' side='right'caption='[[8cxp]], [[Resolution|resolution]] 2.47Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8cxp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Senecavirus_A Senecavirus A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CXP FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cxp OCA], [https://pdbe.org/8cxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cxp RCSB], [https://www.ebi.ac.uk/pdbsum/8cxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cxp ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/A0A649YC68_9PICO A0A649YC68_9PICO]] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Seneca Valley virus (SVV) is a newly discovered picornavirus in the Senecavirus genus. SVV-001 strain has shown promise as an oncolytic virus against tumors with neuroendocrine features. There is a need to use a structure-based approach to develop virus-like particles capable to mimicking the architecture of naturally occurring empty capsids that can be used as vaccines or as carriers for targeted cancer treatment. However, these empty capsids are inherently less stable, and tedious to purify. This warrants investigation into factors which confer the SVV capsid stability and into combining this knowledge to recombinantly express stable SVV VLPs. In this study, we isolated a thermostable mutant of SVV by thermal selection assays and we characterized a single mutation located in a capsid protein. The cryo-EM map of this mutant showed conformational shifts that facilitated the formation of additional hydrogen bonds and aromatic interactions, which could serve as capsid stabilizing factors. | ||
| - | + | Characterisation of a Seneca Valley virus thermostable mutant.,Jayawardena N, McCarthy C, Wang I, Waqqar S, Burga LN, Strauss M, Bostina M Virology. 2022 Oct;575:74-82. doi: 10.1016/j.virol.2022.08.015. Epub 2022 Aug 30. PMID:36084546<ref>PMID:36084546</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8cxp" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Senecavirus A]] | ||
| + | [[Category: Bostina M]] | ||
| + | [[Category: Jayawardena N]] | ||
| + | [[Category: Strauss M]] | ||
Revision as of 06:22, 28 September 2022
Characterisation of a Seneca Valley Virus Thermostable Mutant
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