7tyb
From Proteopedia
(Difference between revisions)
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==Salicylate Adenylate PchD from Pseudomonas aeruginosa containing salicyl-AMS== | ==Salicylate Adenylate PchD from Pseudomonas aeruginosa containing salicyl-AMS== | ||
| - | <StructureSection load='7tyb' size='340' side='right'caption='[[7tyb]]' scene=''> | + | <StructureSection load='7tyb' size='340' side='right'caption='[[7tyb]], [[Resolution|resolution]] 2.11Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TYB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TYB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7tyb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TYB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TYB FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tyb OCA], [https://pdbe.org/7tyb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tyb RCSB], [https://www.ebi.ac.uk/pdbsum/7tyb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tyb ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KT0:5-O-[(2-hydroxybenzoyl)sulfamoyl]adenosine'>KT0</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tyb OCA], [https://pdbe.org/7tyb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tyb RCSB], [https://www.ebi.ac.uk/pdbsum/7tyb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tyb ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/PCHD_PSEAE PCHD_PSEAE]] Involved in the biosynthesis of the siderophore pyochelin (PubMed:8982005). Specifically adenylates salicylate and loads it onto the holo form of PchE via a thioester linkage to the phosphopanthetheine moiety (By similarity). Is also involved in the synthesis of the antifungal antibiotic dihydroaeruginoic acid (Dha or hydroxyphenyl-thiazolinyl-carboxylate), a precursor of pyochelin (PubMed:8982005).[UniProtKB:A0A0H2ZF83]<ref>PMID:8982005</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pseudomonas aeruginosa is an increasingly antibiotic-resistant pathogen that causes severe lung infections, burn wound infections, and diabetic foot infections. P. aeruginosa produces the siderophore pyochelin through the use of a non-ribosomal peptide synthetase (NRPS) biosynthetic pathway. Targeting members of siderophore NRPS proteins is one avenue currently under investigation for the development of new antibiotics against antibiotic-resistant organisms. Here, the crystal structure of the pyochelin adenylation domain PchD is reported. The structure was solved to 2.11 A when co-crystallized with the adenylation inhibitor 5'-O-(N-salicylsulfamoyl)adenosine (salicyl-AMS) and to 1.69 A with a modified version of salicyl-AMS designed to target an active site cysteine (4-cyano-salicyl-AMS). In the structures, PchD adopts the adenylation conformation, similar to that reported for AB3403 from Acinetobacter baumannii. | ||
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| + | Rational inhibitor design for Pseudomonas aeruginosa salicylate adenylation enzyme PchD.,Shelton CL, Meneely KM, Ronnebaum TA, Chilton AS, Riley AP, Prisinzano TE, Lamb AL J Biol Inorg Chem. 2022 May 5. pii: 10.1007/s00775-022-01941-8. doi:, 10.1007/s00775-022-01941-8. PMID:35513576<ref>PMID:35513576</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7tyb" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa PAO1]] | ||
[[Category: Lamb AL]] | [[Category: Lamb AL]] | ||
[[Category: Meneely KM]] | [[Category: Meneely KM]] | ||
[[Category: Shelton CL]] | [[Category: Shelton CL]] | ||
Revision as of 06:28, 28 September 2022
Salicylate Adenylate PchD from Pseudomonas aeruginosa containing salicyl-AMS
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