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| | ==Unphosphorylated STAT3B core protein binding to dsDNA== | | ==Unphosphorylated STAT3B core protein binding to dsDNA== |
| - | <StructureSection load='4e68' size='340' side='right' caption='[[4e68]], [[Resolution|resolution]] 2.58Å' scene=''> | + | <StructureSection load='4e68' size='340' side='right'caption='[[4e68]], [[Resolution|resolution]] 2.58Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4e68]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E68 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4e68]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E68 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bg1|1bg1]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e68 OCA], [https://pdbe.org/4e68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e68 RCSB], [https://www.ebi.ac.uk/pdbsum/4e68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e68 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Aprf, Stat3, STAT3B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e68 OCA], [http://pdbe.org/4e68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e68 RCSB], [http://www.ebi.ac.uk/pdbsum/4e68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e68 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/STAT3_MOUSE STAT3_MOUSE]] Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. STAT3B interacts with the N-terminal part of JUN to activate such promoters in a cooperative way.<ref>PMID:11294897</ref> | + | [[https://www.uniprot.org/uniprot/STAT3_MOUSE STAT3_MOUSE]] Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. STAT3B interacts with the N-terminal part of JUN to activate such promoters in a cooperative way.<ref>PMID:11294897</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Collie, G W]] | + | [[Category: Mus musculus]] |
| - | [[Category: Parkinson, G N]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Shah, R]] | + | [[Category: Collie GW]] |
| - | [[Category: Activator of transcription]] | + | [[Category: Parkinson GN]] |
| - | [[Category: Cytosol]] | + | [[Category: Shah R]] |
| - | [[Category: Duplex dna]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Protein dna complex]]
| + | |
| - | [[Category: Sh2 domain]]
| + | |
| - | [[Category: Signal transducer]]
| + | |
| - | [[Category: Transcription-dna complex]]
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| Structural highlights
Function
[STAT3_MOUSE] Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. STAT3B interacts with the N-terminal part of JUN to activate such promoters in a cooperative way.[1]
Publication Abstract from PubMed
The STAT3 transcription factor plays a central role in a wide range of cancer types where it is over-expressed. Previously, phosphorylation of this protein was thought to be a prerequisite for direct binding to DNA. However, we have now shown complete binding of a purified unphosphorylated STAT3 (uSTAT3) core directly to M67 DNA, the high affinity STAT3 target DNA sequence, by a protein electrophoretic mobility shift assay (PEMSA). Binding to M67 DNA was inhibited by addition of increasing concentrations of a phosphotyrosyl peptide. X-ray crystallography demonstrates one mode of binding that is similar to that known for the STAT3 core phosphorylated at Y705. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: pSTAT3betatcandpSTAT3betatcbindbymolecular sieving(View interaction).
Observation of unphosphorylated STAT3 core protein binding to target dsDNA by PEMSA and X-ray crystallography.,Nkansah E, Shah R, Collie GW, Parkinson GN, Palmer J, Rahman KM, Bui TT, Drake AF, Husby J, Neidle S, Zinzalla G, Thurston DE, Wilderspin AF FEBS Lett. 2013 Feb 19. pii: S0014-5793(13)00110-5. doi:, 10.1016/j.febslet.2013.01.065. PMID:23434585[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hart KC, Robertson SC, Donoghue DJ. Identification of tyrosine residues in constitutively activated fibroblast growth factor receptor 3 involved in mitogenesis, Stat activation, and phosphatidylinositol 3-kinase activation. Mol Biol Cell. 2001 Apr;12(4):931-42. PMID:11294897
- ↑ Nkansah E, Shah R, Collie GW, Parkinson GN, Palmer J, Rahman KM, Bui TT, Drake AF, Husby J, Neidle S, Zinzalla G, Thurston DE, Wilderspin AF. Observation of unphosphorylated STAT3 core protein binding to target dsDNA by PEMSA and X-ray crystallography. FEBS Lett. 2013 Feb 19. pii: S0014-5793(13)00110-5. doi:, 10.1016/j.febslet.2013.01.065. PMID:23434585 doi:http://dx.doi.org/10.1016/j.febslet.2013.01.065
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