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| | ==Fab structure of anti-Vaccinia virus D8L antigen mouse IgG2a LA5== | | ==Fab structure of anti-Vaccinia virus D8L antigen mouse IgG2a LA5== |
| - | <StructureSection load='4ebq' size='340' side='right' caption='[[4ebq]], [[Resolution|resolution]] 1.60Å' scene=''> | + | <StructureSection load='4ebq' size='340' side='right'caption='[[4ebq]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ebq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EBQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EBQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ebq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EBQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EBQ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4e9o|4e9o]], [[2i9l|2i9l]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ebq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ebq OCA], [https://pdbe.org/4ebq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ebq RCSB], [https://www.ebi.ac.uk/pdbsum/4ebq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ebq ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IgG2a, VAR-gene, heavy chain ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), IgG2a, VAR-gene, light chain ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ebq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ebq OCA], [http://pdbe.org/4ebq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ebq RCSB], [http://www.ebi.ac.uk/pdbsum/4ebq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ebq ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | ==See Also== | | ==See Also== |
| - | *[[3D structures of monoclonal antibody|3D structures of monoclonal antibody]] | + | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Matho, M H]] | + | [[Category: Mus musculus]] |
| - | [[Category: Zajonc, D M]] | + | [[Category: Matho MH]] |
| - | [[Category: Cdr]] | + | [[Category: Zajonc DM]] |
| - | [[Category: Conformational epitope]]
| + | |
| - | [[Category: Constant domain]]
| + | |
| - | [[Category: D8l antigen binding]]
| + | |
| - | [[Category: D8l envelope protein]]
| + | |
| - | [[Category: D8l vaccinia envelope protein]]
| + | |
| - | [[Category: Discontinuous epitope]]
| + | |
| - | [[Category: Hypervariable region]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Neutralizing antibody]]
| + | |
| - | [[Category: Neutralizing mab in presence of complement]]
| + | |
| - | [[Category: Papain treatment]]
| + | |
| - | [[Category: Vaccinia virus]]
| + | |
| - | [[Category: Variable domain]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Smallpox vaccine is considered a gold standard of vaccines, as it is the only one that has led to the complete eradication of an infectious disease from the human population. B cell responses are critical for the protective immunity induced by the vaccine, yet their targeted epitopes recognized in humans remain poorly described. Here we describe the biochemical and structural characterization of one of the immunodominant vaccinia virus (VACV) antigens D8 and its binding to the monoclonal antibody LA5, which is capable of neutralizing VACV in the presence of complement. Full length D8 ectodomain was found to form a tetramer. We determined the crystal structure of the LA5 Fab - monomeric D8 complex at a resolution of 2.1 A, as well as the unliganded structures of D8 and LA5-Fab at resolutions of 1.42 A and 1.6 A, respectively. D8 features a carbonic anhydrase (CAH) fold that has evolved to bind to the glycosaminoglycan (GAG) chondroitin sulfate (CS) on host cells. The central positively charged crevice of D8 was predicted as the CS binding site by automated docking experiments. Furthermore, sequence alignment of various poxvirus D8 orthologs revealed that this crevice is structurally conserved. The D8 epitope is formed by 23 discontinuous residues that are spread across 80% of the D8 protein sequence. Interestingly, LA5 binds with a high affinity lock-and-key mechanism above this crevice with an unusually large antibody - antigen interface, burying 2434 A(2) of protein surface.
Structural and biochemical characterization of the vaccinia virus envelope protein D8 and its recognition by the antibody LA5.,Matho MH, Maybeno M, Benhnia MR, Becker D, Meng X, Xiang Y, Crotty S, Peters B, Zajonc DM J Virol. 2012 May 23. PMID:22623786[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Matho MH, Maybeno M, Benhnia MR, Becker D, Meng X, Xiang Y, Crotty S, Peters B, Zajonc DM. Structural and biochemical characterization of the vaccinia virus envelope protein D8 and its recognition by the antibody LA5. J Virol. 2012 May 23. PMID:22623786 doi:10.1128/JVI.00836-12
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