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| | ==Crystal structure of Autophagic E2, Atg10== | | ==Crystal structure of Autophagic E2, Atg10== |
| - | <StructureSection load='4ebr' size='340' side='right' caption='[[4ebr]], [[Resolution|resolution]] 2.70Å' scene=''> | + | <StructureSection load='4ebr' size='340' side='right'caption='[[4ebr]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ebr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EBR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EBR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ebr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EBR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ebr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ebr OCA], [https://pdbe.org/4ebr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ebr RCSB], [https://www.ebi.ac.uk/pdbsum/4ebr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ebr ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ATG10, APG10, YLL042C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ebr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ebr OCA], [http://pdbe.org/4ebr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ebr RCSB], [http://www.ebi.ac.uk/pdbsum/4ebr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ebr ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ATG10_YEAST ATG10_YEAST]] E2-like enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for proper localization of ATG8 to the preautophagosomal structure (PAS). Likely serves as an ATG5-recognition molecule. | + | [[https://www.uniprot.org/uniprot/ATG10_YEAST ATG10_YEAST]] E2-like enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for proper localization of ATG8 to the preautophagosomal structure (PAS). Likely serves as an ATG5-recognition molecule. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Autophagy-related protein|Autophagy-related protein]] | + | *[[Autophagy-related protein 3D structures|Autophagy-related protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Hong, S B]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Kim, B W]] | + | [[Category: Hong SB]] |
| - | [[Category: Kim, J H]] | + | [[Category: Kim BW]] |
| - | [[Category: Song, H K]] | + | [[Category: Kim JH]] |
| - | [[Category: Atg12]] | + | [[Category: Song HK]] |
| - | [[Category: Atg3]]
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| - | [[Category: Atg5]]
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| - | [[Category: Atg7]]
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| - | [[Category: Autophagy]]
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| - | [[Category: E2-conjugating enzyme]]
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| - | [[Category: Ligase]]
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| - | [[Category: Protein binding]]
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| - | [[Category: Thiolation]]
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| Structural highlights
Function
[ATG10_YEAST] E2-like enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for proper localization of ATG8 to the preautophagosomal structure (PAS). Likely serves as an ATG5-recognition molecule.
Publication Abstract from PubMed
Autophagy is a regulated degradation pathway that plays a critical role in all eukaryotic life cycles. One interesting feature of the core autophagic process, autophagosome formation, is similar to ubiquitination. One of two autophagic E2 enzymes, Atg10, interacts with Atg7 to receive Atg12, a ubiquitin-like molecule, and is also involved in the Atg12-Atg5 conjugation reaction. To date, no information on the interaction between Atg10 and Atg7 has been reported, although structural information is available pertaining to the individual components. Here, the crystal structure of Atg10 from Saccharomyces cerevisiae is described at 2.7 A resolution. A significant improvement of the diffraction limit by heavy-atom derivatization was essential for structure determination. The core fold of yeast Atg10 is well conserved compared with those of Atg3 and other E2 enzymes. In contrast to other E2 enzymes, however, the autophagic E2 enzymes Atg3 and Atg10 possess insertion regions in the middle of the core fold and may be involved in protein function. The missing segment, which was termed the `FR-region', in Atg10 may be important for interaction with the E1 enzyme Atg7. This study provides a framework for understanding the E2 conjugation reaction in autophagy.
Structure of the autophagic E2 enzyme Atg10.,Hong SB, Kim BW, Kim JH, Song HK Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1409-17. Epub 2012 Sep, 18. PMID:22993095[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hong SB, Kim BW, Kim JH, Song HK. Structure of the autophagic E2 enzyme Atg10. Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1409-17. Epub 2012 Sep, 18. PMID:22993095 doi:10.1107/S0907444912034166
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