1ik6
From Proteopedia
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'''3D structure of the E1beta subunit of pyruvate dehydrogenase from the archeon Pyrobaculum aerophilum''' | '''3D structure of the E1beta subunit of pyruvate dehydrogenase from the archeon Pyrobaculum aerophilum''' | ||
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[[Category: Kleiger, G.]] | [[Category: Kleiger, G.]] | ||
[[Category: Perry, J.]] | [[Category: Perry, J.]] | ||
| - | [[Category: | + | [[Category: E1beta]] |
| - | [[Category: | + | [[Category: Gxxxg]] |
| - | [[Category: | + | [[Category: Pyruvate dehydrogenase]] |
| - | [[Category: | + | [[Category: Tetramer]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:05:23 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:05, 2 May 2008
3D structure of the E1beta subunit of pyruvate dehydrogenase from the archeon Pyrobaculum aerophilum
Overview
As part of a structural genomics project, we have determined the 2.0 A structure of the E1beta subunit of pyruvate dehydrogenase from Pyrobaculum aerophilum (PA), a thermophilic archaeon. The overall fold of E1beta from PA is closely similar to the previously determined E1beta structures from humans (HU) and P. putida (PP). However, unlike the HU and PP structures, the PA structure was determined in the absence of its partner subunit, E1alpha. Significant structural rearrangements occur in E1beta when its E1alpha partner is absent, including rearrangement of several secondary structure elements such as helix C. Helix C is buried by E1alpha in the HU and PP structures, but makes crystal contacts in the PA structure that lead to an apparent beta(4) tetramer. Static light scattering and sedimentation velocity data are consistent with the formation of PA E1beta tetramers in solution. The interaction of helix C with its symmetry-related counterpart stabilizes the tetrameric interface, where two glycine residues on the same face of one helix create a packing surface for the other helix. This GPhiXXG helix-helix interaction motif has previously been found in interacting transmembrane helices, and is found here at the E1alpha-E1beta interface for both the HU and PP alpha(2)beta(2) tetramers. As a case study in structural genomics, this work illustrates that comparative analysis of protein structures can identify the structural significance of a sequence motif.
About this Structure
1IK6 is a Single protein structure of sequence from Pyrobaculum aerophilum. Full crystallographic information is available from OCA.
Reference
3D structure and significance of the GPhiXXG helix packing motif in tetramers of the E1beta subunit of pyruvate dehydrogenase from the archeon Pyrobaculum aerophilum., Kleiger G, Perry J, Eisenberg D, Biochemistry. 2001 Dec 4;40(48):14484-92. PMID:11724561 Page seeded by OCA on Fri May 2 20:05:23 2008
