1ik9
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1ik9.jpg|left|200px]] | [[Image:1ik9.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1ik9", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1ik9| PDB=1ik9 | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''CRYSTAL STRUCTURE OF A XRCC4-DNA LIGASE IV COMPLEX''' | '''CRYSTAL STRUCTURE OF A XRCC4-DNA LIGASE IV COMPLEX''' | ||
| Line 27: | Line 24: | ||
Crystal structure of an Xrcc4-DNA ligase IV complex., Sibanda BL, Critchlow SE, Begun J, Pei XY, Jackson SP, Blundell TL, Pellegrini L, Nat Struct Biol. 2001 Dec;8(12):1015-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11702069 11702069] | Crystal structure of an Xrcc4-DNA ligase IV complex., Sibanda BL, Critchlow SE, Begun J, Pei XY, Jackson SP, Blundell TL, Pellegrini L, Nat Struct Biol. 2001 Dec;8(12):1015-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11702069 11702069] | ||
[[Category: DNA Ligase]] | [[Category: DNA Ligase]] | ||
| - | [[Category: DNA ligase (ATP)]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 37: | Line 33: | ||
[[Category: Pellegrini, L.]] | [[Category: Pellegrini, L.]] | ||
[[Category: Sibanda, B L.]] | [[Category: Sibanda, B L.]] | ||
| - | [[Category: | + | [[Category: Coiled coil]] |
| - | [[Category: | + | [[Category: Dna end joining]] |
| - | [[Category: | + | [[Category: Double-strand break repair]] |
| - | [[Category: | + | [[Category: Protein-protein complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:05:38 2008'' | |
| - | + | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:05, 2 May 2008
CRYSTAL STRUCTURE OF A XRCC4-DNA LIGASE IV COMPLEX
Contents |
Overview
A complex of two proteins, Xrcc4 and DNA ligase IV, plays a fundamental role in DNA non-homologous end joining (NHEJ), a cellular function required for double-strand break repair and V(D)J recombination. Here we report the crystal structure of human Xrcc4 bound to a polypeptide that corresponds to the DNA ligase IV sequence linking its two BRCA1 C-terminal (BRCT) domains. In the complex, a single ligase chain binds asymmetrically to an Xrcc4 dimer. The helical tails of Xrcc4 undergo a substantial conformational change relative to the uncomplexed protein, forming a coiled coil that unwinds upon ligase binding, leading to a flat interaction surface. A buried network of charged hydrogen bonds surrounded by extensive hydrophobic contacts explains the observed tightness of the interaction. The strong conservation of residues at the interface between the two proteins provides evidence that the observed mode of interaction has been maintained in NHEJ throughout evolution.
Disease
Known disease associated with this structure: LIG4 syndrome OMIM:[601837], Multiple myeloma, resistance to OMIM:[601837]
About this Structure
1IK9 is a Protein complex structure of sequences from Homo sapiens. The following page contains interesting information on the relation of 1IK9 with [DNA Ligase]. Full crystallographic information is available from OCA.
Reference
Crystal structure of an Xrcc4-DNA ligase IV complex., Sibanda BL, Critchlow SE, Begun J, Pei XY, Jackson SP, Blundell TL, Pellegrini L, Nat Struct Biol. 2001 Dec;8(12):1015-9. PMID:11702069 Page seeded by OCA on Fri May 2 20:05:38 2008
