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| | ==Staphylococcus epidermidis TcaR in complex with RNA== | | ==Staphylococcus epidermidis TcaR in complex with RNA== |
| - | <StructureSection load='4ejt' size='340' side='right' caption='[[4ejt]], [[Resolution|resolution]] 3.00Å' scene=''> | + | <StructureSection load='4ejt' size='340' side='right'caption='[[4ejt]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ejt]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Staeq Staeq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EJT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ejt]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_epidermidis_RP62A Staphylococcus epidermidis RP62A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EJT FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4eju|4eju]], [[4ejv|4ejv]], [[4ejw|4ejw]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ejt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ejt OCA], [https://pdbe.org/4ejt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ejt RCSB], [https://www.ebi.ac.uk/pdbsum/4ejt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ejt ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tcaR, SERP1949 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176279 STAEQ])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ejt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ejt OCA], [http://pdbe.org/4ejt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ejt RCSB], [http://www.ebi.ac.uk/pdbsum/4ejt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ejt ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [[https://www.uniprot.org/uniprot/Q5HLN6_STAEQ Q5HLN6_STAEQ]] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Staeq]] | + | [[Category: Large Structures]] |
| - | [[Category: Chang, Y M]] | + | [[Category: Staphylococcus epidermidis RP62A]] |
| - | [[Category: Chen, C K.M]] | + | [[Category: Chang YM]] |
| - | [[Category: Wang, A H.J]] | + | [[Category: Chen CK-M]] |
| - | [[Category: Marr family protein]] | + | [[Category: Wang AH-J]] |
| - | [[Category: Tcar]]
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| - | [[Category: Transcription regulator-rna complex]]
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| Structural highlights
Function
[Q5HLN6_STAEQ]
Publication Abstract from PubMed
The negative transcription regulator of the ica locus, TcaR, regulates proteins involved in the biosynthesis of poly-N-acetylglucosamine (PNAG). Absence of TcaR increases PNAG production and promotes biofilm formation in Staphylococci. Previously, the 3D structure of TcaR in its apo form and its complex structure with several antibiotics have been analyzed. However, the detailed mechanism of multiple antibiotic resistance regulator (MarR) family proteins such as TcaR is unclear and only restricted on the binding ability of double-strand DNA (dsDNA). Here we show by electrophoretic mobility shift assay (EMSA), electron microscopy (EM), circular dichroism (CD), and Biacore analysis that TcaR can interact strongly with single-stranded DNA (ssDNA), thereby identifying a new role in MarR family proteins. Moreover, we show that TcaR preferentially binds 33-mer ssDNA over double-stranded DNA and inhibits viral ssDNA replication. In contrast, such ssDNA binding properties were not observed for other MarR family protein and TetR family protein, suggesting that the results from our studies are not an artifact due to simple charge interactions between TcaR and ssDNA. Overall, these results suggest a novel role for TcaR in regulation of DNA replication. We anticipate that the results of this work will extend our understanding of MarR family protein and broaden the development of new therapeutic strategies for Staphylococci.
Functional studies of ssDNA binding ability of MarR family protein TcaR from Staphylococcus epidermidis.,Chang YM, Chen CK, Chang YC, Jeng WY, Hou MH, Wang AH PLoS One. 2012;7(9):e45665. doi: 10.1371/journal.pone.0045665. Epub 2012 Sep 21. PMID:23029170[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chang YM, Chen CK, Chang YC, Jeng WY, Hou MH, Wang AH. Functional studies of ssDNA binding ability of MarR family protein TcaR from Staphylococcus epidermidis. PLoS One. 2012;7(9):e45665. doi: 10.1371/journal.pone.0045665. Epub 2012 Sep 21. PMID:23029170 doi:10.1371/journal.pone.0045665
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