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| | ==Crystal structure of SpLsm4== | | ==Crystal structure of SpLsm4== |
| - | <StructureSection load='4emh' size='340' side='right' caption='[[4emh]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='4emh' size='340' side='right'caption='[[4emh]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4emh]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EMH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EMH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4emh]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EMH FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4emg|4emg]], [[4emk|4emk]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4emh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4emh OCA], [https://pdbe.org/4emh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4emh RCSB], [https://www.ebi.ac.uk/pdbsum/4emh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4emh ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lsm4, SPBC30D10.06 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4emh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4emh OCA], [http://pdbe.org/4emh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4emh RCSB], [http://www.ebi.ac.uk/pdbsum/4emh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4emh ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LSM4_SCHPO LSM4_SCHPO]] Binds specifically to the 3'-terminal U-tract of U6 snRNA. | + | [[https://www.uniprot.org/uniprot/LSM4_SCHPO LSM4_SCHPO]] Binds specifically to the 3'-terminal U-tract of U6 snRNA. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fission yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Jiang, S M]] | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: Song, H W]] | + | [[Category: Jiang SM]] |
| - | [[Category: Wu, D H]] | + | [[Category: Song HW]] |
| - | [[Category: Lsm protein]] | + | [[Category: Wu DH]] |
| - | [[Category: Mrna decay]]
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| - | [[Category: Pre-mrna splicing]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Sm fold]]
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| Structural highlights
Function
[LSM4_SCHPO] Binds specifically to the 3'-terminal U-tract of U6 snRNA.
Publication Abstract from PubMed
Sm-like (Lsm) proteins are ubiquitous and function in many aspects of RNA metabolism, including pre-mRNA splicing, nuclear RNA processing, mRNA decay and miRNA biogenesis. Here three crystal structures including Lsm3, Lsm4 and Lsm5/6/7 sub-complex from S. pombe are reported. These structures show that all the five individual Lsm subunits share a conserved Sm fold, and Lsm3, Lsm4, and Lsm5/6/7 form a heptamer, a trimer and a hexamer within the crystal lattice, respectively. Analytical ultracentrifugation indicates that Lsm3 and Lsm5/6/7 sub-complex exist in solution as a heptamer and a hexamer, respectively while Lsm4 undergoes a dynamic equilibrium between monomer and trimer in solution. RNA binding assays show that Lsm2/3 and Lsm5/6/7 bind to oligo(U) whereas no RNA binding is observed for Lsm3 and Lsm4. Analysis of the inter-subunit interactions in Lsm5/6/7 reveals the organization order among Lsm5, Lsm6 and Lsm7.
Crystal Structures of Lsm3, Lsm4 and Lsm5/6/7 from Schizosaccharomyces pombe.,Wu D, Jiang S, Bowler MW, Song H PLoS One. 2012;7(5):e36768. Epub 2012 May 17. PMID:22615807[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wu D, Jiang S, Bowler MW, Song H. Crystal Structures of Lsm3, Lsm4 and Lsm5/6/7 from Schizosaccharomyces pombe. PLoS One. 2012;7(5):e36768. Epub 2012 May 17. PMID:22615807 doi:10.1371/journal.pone.0036768
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