Sandbox reserved 1753
From Proteopedia
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== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
A specific DNA complex of the 65-residue, N-terminal fragment of the yeast transcriptional activator, GAL4, has been analysed at 2.7 A resolution by X-ray crystallography. The protein binds as a <scene name='92/927197/Dimer/8'>Dimer</scene> to a symmetrical 17-base-pair sequence. Each subunit folds into three distinct modules: a compact, <scene name='92/927197/Dimer_metal_binding/1'>Dimer Metal binding</scene> (residues 8-40), an <scene name='92/927197/Dimer_extended_linker/1'>Dimer extended Linker</scene> (41-49), and | A specific DNA complex of the 65-residue, N-terminal fragment of the yeast transcriptional activator, GAL4, has been analysed at 2.7 A resolution by X-ray crystallography. The protein binds as a <scene name='92/927197/Dimer/8'>Dimer</scene> to a symmetrical 17-base-pair sequence. Each subunit folds into three distinct modules: a compact, <scene name='92/927197/Dimer_metal_binding/1'>Dimer Metal binding</scene> (residues 8-40), an <scene name='92/927197/Dimer_extended_linker/1'>Dimer extended Linker</scene> (41-49), and | ||
| - | an <scene name='92/927197/Dimer_alpha_helicaldimerzation/1'>alpha-helicaldimerization element</scene> (50-64). A small, | + | an <scene name='92/927197/Dimer_alpha_helicaldimerzation/1'>alpha-helicaldimerization element</scene> (50-64). A small, <scene name='92/927197/Dimer_zn2/1'>Dimer ZN2</scene>-containing domain recognizes a conserved CCG triplet at each end of the site through direct contacts with the major groove. A short coiled-coil dimerization element imposes 2-fold symmetry. A segment of extended polypeptide chain links the metal-binding module to the dimerization element and specifies the length of the site. The relatively open structure of the complex would allow another protein to bind coordinately with GAL4. |
DNA recognition by GAL4: structure of a protein-DNA complex.,Marmorstein R, Carey M, Ptashne M, Harrison SC Nature. 1992 Apr 2;356(6368):408-14. PMID:1557122<ref>PMID:1557122</ref> | DNA recognition by GAL4: structure of a protein-DNA complex.,Marmorstein R, Carey M, Ptashne M, Harrison SC Nature. 1992 Apr 2;356(6368):408-14. PMID:1557122<ref>PMID:1557122</ref> | ||
Revision as of 17:41, 2 October 2022
==DNA RECOGNITION BY GAL4: STRUCTURE OF A PROTEIN/DNA COMPLEX==
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