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4esw

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Revision as of 04:13, 7 October 2022

Crystal structure of C. albicans Thi5 H66G mutant

Structural highlights

4esw is a 2 chain structure with sequence from Candida albicans WO-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

THI5_CANAW Responsible for the formation of the pyrimidine heterocycle in the thiamine biosynthesis pathway. Catalyzes the formation of hydroxymethylpyrimidine phosphate (HMP-P) from histidine and pyridoxal phosphate (PLP). The protein uses PLP and the active site histidine to form HMP-P, generating an inactive enzyme. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme.^[1]

Publication Abstract from PubMed

In Saccharomyces cerevisiae , thiamin pyrimidine is formed from histidine and pyridoxal phosphate (PLP). The origin of all of the pyrimidine atoms has been previously determined using labeling studies and suggests that the pyrimidine is formed using remarkable chemistry that is without chemical or biochemical precedent. Here we report the overexpression of the closely related Candida albicans pyrimidine synthase (THI5p) and the reconstitution and preliminary characterization of the enzymatic activity. A structure of the C. albicans THI5p shows PLP bound at the active site via an imine with Lys62 and His66 in close proximity to the PLP. Our data suggest that His66 of the THI5 protein is the histidine source for pyrimidine formation and that the pyrimidine synthase is a single-turnover enzyme.

Thiamin pyrimidine biosynthesis in Candida albicans : a remarkable reaction between histidine and pyridoxal phosphate.,Lai RY, Huang S, Fenwick MK, Hazra A, Zhang Y, Rajashankar K, Philmus B, Kinsland C, Sanders JM, Ealick SE, Begley TP J Am Chem Soc. 2012 Jun 6;134(22):9157-9. Epub 2012 May 24. PMID:22568620^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lai RY, Huang S, Fenwick MK, Hazra A, Zhang Y, Rajashankar K, Philmus B, Kinsland C, Sanders JM, Ealick SE, Begley TP. Thiamin pyrimidine biosynthesis in Candida albicans : a remarkable reaction between histidine and pyridoxal phosphate. J Am Chem Soc. 2012 Jun 6;134(22):9157-9. Epub 2012 May 24. PMID:22568620 doi:http://dx.doi.org/10.1021/ja302474a
↑ Lai RY, Huang S, Fenwick MK, Hazra A, Zhang Y, Rajashankar K, Philmus B, Kinsland C, Sanders JM, Ealick SE, Begley TP. Thiamin pyrimidine biosynthesis in Candida albicans : a remarkable reaction between histidine and pyridoxal phosphate. J Am Chem Soc. 2012 Jun 6;134(22):9157-9. Epub 2012 May 24. PMID:22568620 doi:http://dx.doi.org/10.1021/ja302474a

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4esw"

@@ Line 1: / Line 1: @@
 ==Crystal structure of C. albicans Thi5 H66G mutant==
-<StructureSection load='4esw' size='340' side='right' caption='[[4esw]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+<StructureSection load='4esw' size='340' side='right'caption='[[4esw]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4esw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canaw Canaw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ESW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ESW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4esw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_WO-1 Candida albicans WO-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ESW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ESW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4esx|4esx]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4esw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4esw OCA], [https://pdbe.org/4esw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4esw RCSB], [https://www.ebi.ac.uk/pdbsum/4esw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4esw ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CAWG_02199 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294748 CANAW])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4esw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4esw OCA], [http://pdbe.org/4esw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4esw RCSB], [http://www.ebi.ac.uk/pdbsum/4esw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4esw ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/THI5_CANAW THI5_CANAW] Responsible for the formation of the pyrimidine heterocycle in the thiamine biosynthesis pathway. Catalyzes the formation of hydroxymethylpyrimidine phosphate (HMP-P) from histidine and pyridoxal phosphate (PLP). The protein uses PLP and the active site histidine to form HMP-P, generating an inactive enzyme. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme.<ref>PMID:22568620</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Canaw]]
+[[Category: Candida albicans WO-1]]
-[[Category: Begley, T P]]
+[[Category: Large Structures]]
-[[Category: Ealick, S E]]
+[[Category: Begley TP]]
-[[Category: Fenwick, M K]]
+[[Category: Ealick SE]]
-[[Category: Hazra, A]]
+[[Category: Fenwick MK]]
-[[Category: Huang, S]]
+[[Category: Hazra A]]
-[[Category: Kinsland, C]]
+[[Category: Huang S]]
-[[Category: Lai, R]]
+[[Category: Kinsland C]]
-[[Category: Philmus, B]]
+[[Category: Lai R]]
-[[Category: Rajashankar, K]]
+[[Category: Philmus B]]
-[[Category: Sanders, J]]
+[[Category: Rajashankar K]]
-[[Category: Zhang, Y]]
+[[Category: Sanders J]]
-[[Category: Thiamin pyrimidine biosynthesis]]
+[[Category: Zhang Y]]
-[[Category: Transferase]]

4esw

From Proteopedia

Revision as of 04:13, 7 October 2022

Crystal structure of C. albicans Thi5 H66G mutant

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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