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1ilw

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[[Image:1ilw.gif|left|200px]]
[[Image:1ilw.gif|left|200px]]
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{{Structure
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|PDB= 1ilw |SIZE=350|CAPTION= <scene name='initialview01'>1ilw</scene>, resolution 2.05&Aring;
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The line below this paragraph, containing "STRUCTURE_1ilw", creates the "Structure Box" on the page.
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nicotinamidase Nicotinamidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.19 3.5.1.19] </span>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd01011 nicotinamidase]</span>
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{{STRUCTURE_1ilw| PDB=1ilw | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ilw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ilw OCA], [http://www.ebi.ac.uk/pdbsum/1ilw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ilw RCSB]</span>
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'''Crystal Structure of Pyrazinamidase/Nicotinamidase of Pyrococcus horikoshii'''
'''Crystal Structure of Pyrazinamidase/Nicotinamidase of Pyrococcus horikoshii'''
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[[Category: Du, X.]]
[[Category: Du, X.]]
[[Category: Kim, S H.]]
[[Category: Kim, S H.]]
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[[Category: amidase]]
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[[Category: Amidase]]
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[[Category: berkeley structural genomics center]]
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[[Category: Berkeley structural genomics center]]
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[[Category: bsgc structure funded by nih]]
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[[Category: Bsgc structure funded by nih]]
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[[Category: cysteine hydrolase]]
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[[Category: Cysteine hydrolase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: nicotinamidase]]
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[[Category: Nicotinamidase]]
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[[Category: protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: psi]]
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[[Category: Psi]]
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[[Category: pyrazinamidase]]
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[[Category: Pyrazinamidase]]
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[[Category: pyrazinamide]]
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[[Category: Pyrazinamide]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: tuberculosis]]
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[[Category: Tuberculosis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:08:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:21:36 2008''
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Revision as of 17:08, 2 May 2008

Image:1ilw.gif

Template:STRUCTURE 1ilw

Crystal Structure of Pyrazinamidase/Nicotinamidase of Pyrococcus horikoshii


Overview

Bacterial pyrazinamidase (PZAase)/nicotinamidase converts pyrazinamide (PZA) to ammonia and pyrazinoic acid, which is active against Mycobacterium tuberculosis. Loss of PZAase activity is the major mechanism of pyrazinamide-resistance by M. tuberculosis. We have determined the crystal structure of the gene product of Pyrococcus horikoshii 999 (PH999), a PZAase, and its complex with zinc ion by X-ray crystallography. The overall fold of PH999 is similar to that of N-carbamoylsarcosine amidohydrolase (CSHase) of Arthrobacter sp. and YcaC of Escherichia coli, a protein with unknown physiological function. The active site of PH999 was identified by structural features that are also present in the active sites of CSHase and YcaC: a triad (D10, K94, and C133) and a cis-peptide (between V128 and A129). Surprisingly, a metal ion-binding site was revealed in the active site and subsequently confirmed by crystal structure of PH999 in complex with Zn(2+). The roles of the triad, cis-peptide, and metal ion in the catalysis are proposed. Because of extensive homology between PH999 and PZAase of M. tuberculosis (37% sequence identity), the structure of PH999 provides a structural basis for understanding PZA-resistance by M. tuberculosis harboring PZAase mutations.

About this Structure

1ILW is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.

Reference

Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii., Du X, Wang W, Kim R, Yakota H, Nguyen H, Kim SH, Biochemistry. 2001 Nov 27;40(47):14166-72. PMID:11714269 Page seeded by OCA on Fri May 2 20:08:18 2008

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