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| | ==Structure of the SH3 domain of human nebulette in complex with a peptide of XIRP2== | | ==Structure of the SH3 domain of human nebulette in complex with a peptide of XIRP2== |
| - | <StructureSection load='4f14' size='340' side='right' caption='[[4f14]], [[Resolution|resolution]] 1.20Å' scene=''> | + | <StructureSection load='4f14' size='340' side='right'caption='[[4f14]], [[Resolution|resolution]] 1.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4f14]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F14 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F14 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4f14]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F14 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NEBL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f14 OCA], [https://pdbe.org/4f14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f14 RCSB], [https://www.ebi.ac.uk/pdbsum/4f14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f14 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f14 OCA], [http://pdbe.org/4f14 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f14 RCSB], [http://www.ebi.ac.uk/pdbsum/4f14 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f14 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NEBL_HUMAN NEBL_HUMAN]] Binds to actin and plays an important role in the assembly of the Z-disk. Isoform 2 might play a role in the assembly of focal adhesion.<ref>PMID:15004028</ref> [[http://www.uniprot.org/uniprot/XIRP2_HUMAN XIRP2_HUMAN]] Protects actin filaments from depolymerization. | + | [https://www.uniprot.org/uniprot/NEBL_HUMAN NEBL_HUMAN] Binds to actin and plays an important role in the assembly of the Z-disk. Isoform 2 might play a role in the assembly of focal adhesion.<ref>PMID:15004028</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Sauer, F]] | + | [[Category: Large Structures]] |
| - | [[Category: Vahokoski, J]] | + | [[Category: Sauer F]] |
| - | [[Category: Wilmanns, M]] | + | [[Category: Vahokoski J]] |
| - | [[Category: Actin-binding protein-peptide complex]] | + | [[Category: Wilmanns M]] |
| - | [[Category: Heart muscle]]
| + | |
| - | [[Category: Sh3 domain]]
| + | |
| Structural highlights
Function
NEBL_HUMAN Binds to actin and plays an important role in the assembly of the Z-disk. Isoform 2 might play a role in the assembly of focal adhesion.[1]
Publication Abstract from PubMed
The Xin actin-binding repeat-containing proteins Xin and XIRP2 are exclusively expressed in striated muscle cells, where they are believed to play an important role in development. In adult muscle, both proteins are concentrated at attachment sites of myofibrils to the membrane. In contrast, during development they are localized to immature myofibrils together with their binding partner, filamin C, indicating an involvement of both proteins in myofibril assembly. We identify the SH3 domains of nebulin and nebulette as novel ligands of proline-rich regions of Xin and XIRP2. Precise binding motifs are mapped and shown to bind both SH3 domains with micromolar affinity. Cocrystallization of the nebulette SH3 domain with the interacting XIRP2 peptide PPPTLPKPKLPKH reveals selective interactions that conform to class II SH3 domain-binding peptides. Bimolecular fluorescence complementation experiments in cultured muscle cells indicate a temporally restricted interaction of Xin-repeat proteins with nebulin/nebulette during early stages of myofibril development that is lost upon further maturation. In mature myofibrils, this interaction is limited to longitudinally oriented structures associated with myofibril development and remodeling. These data provide new insights into the role of Xin actin-binding repeat-containing proteins (together with their interaction partners) in myofibril assembly and after muscle damage.
Identification of Xin-repeat proteins as novel ligands of the SH3 domains of nebulin and nebulette and analysis of their interaction during myofibril formation and remodeling.,Eulitz S, Sauer F, Pelissier MC, Boisguerin P, Molt S, Schuld J, Orfanos Z, Kley RA, Volkmer R, Wilmanns M, Kirfel G, van der Ven PF, Furst DO Mol Biol Cell. 2013 Oct;24(20):3215-26. doi: 10.1091/mbc.E13-04-0202. Epub 2013, Aug 28. PMID:23985323[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li B, Zhuang L, Trueb B. Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1. J Biol Chem. 2004 May 7;279(19):20401-10. Epub 2004 Mar 5. PMID:15004028 doi:10.1074/jbc.M310304200
- ↑ Eulitz S, Sauer F, Pelissier MC, Boisguerin P, Molt S, Schuld J, Orfanos Z, Kley RA, Volkmer R, Wilmanns M, Kirfel G, van der Ven PF, Furst DO. Identification of Xin-repeat proteins as novel ligands of the SH3 domains of nebulin and nebulette and analysis of their interaction during myofibril formation and remodeling. Mol Biol Cell. 2013 Oct;24(20):3215-26. doi: 10.1091/mbc.E13-04-0202. Epub 2013, Aug 28. PMID:23985323 doi:http://dx.doi.org/10.1091/mbc.E13-04-0202
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