This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4f2e

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 04:29, 7 October 2022

Crystal structure of the Streptococcus pneumoniae D39 copper chaperone CupA with Cu(I)

Structural highlights

4f2e is a 1 chain structure with sequence from Streptococcus pneumoniae D39. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A0H2ZPL4_STRP2

Publication Abstract from PubMed

Copper resistance has emerged as an important virulence determinant of microbial pathogens. In Streptococcus pneumoniae, copper resistance is mediated by the copper-responsive repressor CopY, CupA and the copper-effluxing P(1B)-type ATPase CopA. We show here that CupA is a previously uncharacterized cell membrane-anchored Cu(I) chaperone and that a Cu(I) binding-competent, membrane-localized CupA is obligatory for copper resistance. The crystal structures of the soluble domain of CupA and the N-terminal metal-binding domain (MBD) of CopA (CopA(MBD)) reveal isostructural cupredoxin-like folds that each harbor a binuclear Cu(I) cluster unprecedented in bacterial copper trafficking. NMR studies reveal unidirectional Cu(I) transfer from the low-affinity site on the soluble domain of CupA to the high-affinity site of CopA(MBD). However, copper binding by CopA(MBD) is not essential for cellular copper resistance, consistent with a primary role of CupA in cytoplasmic Cu(I) sequestration and/or direct delivery to the transmembrane site of CopA for cellular efflux.

A new structural paradigm in copper resistance in Streptococcus pneumoniae.,Fu Y, Tsui HC, Bruce KE, Sham LT, Higgins KA, Lisher JP, Kazmierczak KM, Maroney MJ, Dann CE 3rd, Winkler ME, Giedroc DP Nat Chem Biol. 2013 Jan 27. doi: 10.1038/nchembio.1168. PMID:23354287^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fu Y, Tsui HC, Bruce KE, Sham LT, Higgins KA, Lisher JP, Kazmierczak KM, Maroney MJ, Dann CE 3rd, Winkler ME, Giedroc DP. A new structural paradigm in copper resistance in Streptococcus pneumoniae. Nat Chem Biol. 2013 Jan 27. doi: 10.1038/nchembio.1168. PMID:23354287 doi:http://dx.doi.org/10.1038/nchembio.1168

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4f2e"

Categories: Large Structures | Streptococcus pneumoniae D39 | Dann III CE | Fu Y | Giedroc DP

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Streptococcus pneumoniae D39 copper chaperone CupA with Cu(I)==
-<StructureSection load='4f2e' size='340' side='right' caption='[[4f2e]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
+<StructureSection load='4f2e' size='340' side='right'caption='[[4f2e]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4f2e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strp2 Strp2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F2E FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4f2e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_D39 Streptococcus pneumoniae D39]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F2E FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4f2f|4f2f]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2e OCA], [https://pdbe.org/4f2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f2e RCSB], [https://www.ebi.ac.uk/pdbsum/4f2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f2e ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPD0634, SPD_0634 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=373153 STRP2])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2e OCA], [http://pdbe.org/4f2e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f2e RCSB], [http://www.ebi.ac.uk/pdbsum/4f2e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f2e ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0H2ZPL4_STRP2 A0A0H2ZPL4_STRP2]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Strp2]]
+[[Category: Large Structures]]
-[[Category: Fu, Y]]
+[[Category: Streptococcus pneumoniae D39]]
-[[Category: Giedroc, D P]]
+[[Category: Dann III CE]]
-[[Category: III, C E.Dann]]
+[[Category: Fu Y]]
-[[Category: Cupredoxin fold]]
+[[Category: Giedroc DP]]
-[[Category: Metal transport]]

4f2e

From Proteopedia

Revision as of 04:29, 7 October 2022

Crystal structure of the Streptococcus pneumoniae D39 copper chaperone CupA with Cu(I)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox