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| | ==Crystal structure of the Pseudomonas fluorescens agglutinin (PFA)== | | ==Crystal structure of the Pseudomonas fluorescens agglutinin (PFA)== |
| - | <StructureSection load='4fbo' size='340' side='right' caption='[[4fbo]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='4fbo' size='340' side='right'caption='[[4fbo]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fbo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psepf Psepf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FBO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FBO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fbo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens_Pf0-1 Pseudomonas fluorescens Pf0-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FBO FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3s5v|3s5v]], [[3obl|3obl]], [[4fbr|4fbr]], [[4fbv|4fbv]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fbo OCA], [https://pdbe.org/4fbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fbo RCSB], [https://www.ebi.ac.uk/pdbsum/4fbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fbo ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Pfl01_0508 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=205922 PSEPF])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fbo OCA], [http://pdbe.org/4fbo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fbo RCSB], [http://www.ebi.ac.uk/pdbsum/4fbo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fbo ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q3KJ04_PSEPF Q3KJ04_PSEPF] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4fbo" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4fbo" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Agglutinin 3D structures|Agglutinin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Psepf]] | + | [[Category: Large Structures]] |
| - | [[Category: Gronenborn, A M]] | + | [[Category: Pseudomonas fluorescens Pf0-1]] |
| - | [[Category: Koharudin, L M.I]]
| + | [[Category: Gronenborn AM]] |
| - | [[Category: Beta-barrel]]
| + | [[Category: Koharudin LMI]] |
| - | [[Category: Carbohydrate binding protein]] | + | |
| - | [[Category: Hiv-inactivating]] | + | |
| Structural highlights
Function
Q3KJ04_PSEPF
Publication Abstract from PubMed
Oscillatoria agardhii agglutinin homolog (OAAH) proteins belong to a recently discovered lectin family. All members contain a sequence repeat of approximately 66 amino acids, with the number of repeats varying among different family members. Apart from data for the founding member OAA, neither three-dimensional structures, information about carbohydrate binding specificities, nor antiviral activity data have been available up to now for any other members of the OAAH family. To elucidate the structural basis for the antiviral mechanism of OAAHs, we determined the crystal structures of Pseudomonas fluorescens and Myxococcus xanthus lectins. Both proteins exhibit the same fold, resembling the founding family member, OAA, with minor differences in loop conformations. Carbohydrate binding studies by NMR and x-ray structures of glycan-lectin complexes reveal that the number of sugar binding sites corresponds to the number of sequence repeats in each protein. As for OAA, tight and specific binding to alpha3,alpha6-mannopentaose was observed. All the OAAH proteins described here exhibit potent anti-HIV activity at comparable levels. Altogether, our results provide structural details of the protein-carbohydrate interaction for this novel lectin family and insights into the molecular basis of their HIV inactivation properties.
Structural Insights into the Anti-HIV Activity of the Oscillatoria agardhii Agglutinin Homolog Lectin Family.,Koharudin LM, Kollipara S, Aiken C, Gronenborn AM J Biol Chem. 2012 Sep 28;287(40):33796-811. Epub 2012 Aug 4. PMID:22865886[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Koharudin LM, Kollipara S, Aiken C, Gronenborn AM. Structural Insights into the Anti-HIV Activity of the Oscillatoria agardhii Agglutinin Homolog Lectin Family. J Biol Chem. 2012 Sep 28;287(40):33796-811. Epub 2012 Aug 4. PMID:22865886 doi:http://dx.doi.org/10.1074/jbc.M112.388579
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