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| | ==Crystal structure of an unfused Mre11-Nbs1 complex with two manganese ions per active site== | | ==Crystal structure of an unfused Mre11-Nbs1 complex with two manganese ions per active site== |
| - | <StructureSection load='4fbw' size='340' side='right' caption='[[4fbw]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='4fbw' size='340' side='right'caption='[[4fbw]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fbw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FBW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FBW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fbw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FBW FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fbk|4fbk]], [[4fbq|4fbq]], [[4fcx|4fcx]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fbw OCA], [https://pdbe.org/4fbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fbw RCSB], [https://www.ebi.ac.uk/pdbsum/4fbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fbw ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rad32, SPAC13C5.07 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), nbs1, SPBC6B1.09c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fbw OCA], [http://pdbe.org/4fbw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fbw RCSB], [http://www.ebi.ac.uk/pdbsum/4fbw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fbw ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RAD32_SCHPO RAD32_SCHPO]] Required for the repair of double strand breaks (DSB) caused by gamma and UV radiation. May work in conjunction with rhp51. [[http://www.uniprot.org/uniprot/NBS1_SCHPO NBS1_SCHPO]] Required for DNA damage repair and S-phase DNA damage checkpoint. Involved in telomere length maintenance and maintenance of chromatin structure.<ref>PMID:12944481</ref> <ref>PMID:12944482</ref> | + | [https://www.uniprot.org/uniprot/RAD32_SCHPO RAD32_SCHPO] Required for the repair of double strand breaks (DSB) caused by gamma and UV radiation. May work in conjunction with rhp51. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fission yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Hopfner, K P]] | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: Lammens, K]]
| + | [[Category: Hopfner KP]] |
| - | [[Category: Schiller, C B]]
| + | [[Category: Lammens K]] |
| - | [[Category: Dna double-strand break repair]]
| + | [[Category: Schiller CB]] |
| - | [[Category: Hydrolase]] | + | |
| - | [[Category: Hydrolase-protein binding complex]] | + | |
| - | [[Category: Nuclease]] | + | |
| Structural highlights
Function
RAD32_SCHPO Required for the repair of double strand breaks (DSB) caused by gamma and UV radiation. May work in conjunction with rhp51.
Publication Abstract from PubMed
The Mre11-Rad50-Nbs1 (MRN) complex tethers, processes and signals DNA double-strand breaks, promoting genomic stability. To understand the functional architecture of MRN, we determined the crystal structures of the Schizosaccharomyces pombe Mre11 dimeric catalytic domain alone and in complex with a fragment of Nbs1. Two Nbs1 subunits stretch around the outside of the nuclease domains of Mre11, with one subunit additionally bridging and locking the Mre11 dimer via a highly conserved asymmetrical binding motif. Our results show that Mre11 forms a flexible dimer and suggest that Nbs1 not only is a checkpoint adaptor but also functionally influences Mre11-Rad50. Clinical mutations in Mre11 are located along the Nbs1-interaction sites and weaken the Mre11-Nbs1 interaction. However, they differentially affect DNA repair and telomere maintenance in Saccharomyces cerevisiae, potentially providing insight into their different human disease pathologies.
Structure of Mre11-Nbs1 complex yields insights into ataxia-telangiectasia-like disease mutations and DNA damage signaling.,Schiller CB, Lammens K, Guerini I, Coordes B, Feldmann H, Schlauderer F, Mockel C, Schele A, Strasser K, Jackson SP, Hopfner KP Nat Struct Mol Biol. 2012 Jun 17;19(7):693-700. doi: 10.1038/nsmb.2323. PMID:22705791[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Schiller CB, Lammens K, Guerini I, Coordes B, Feldmann H, Schlauderer F, Mockel C, Schele A, Strasser K, Jackson SP, Hopfner KP. Structure of Mre11-Nbs1 complex yields insights into ataxia-telangiectasia-like disease mutations and DNA damage signaling. Nat Struct Mol Biol. 2012 Jun 17;19(7):693-700. doi: 10.1038/nsmb.2323. PMID:22705791 doi:10.1038/nsmb.2323
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