1in2
From Proteopedia
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'''Peptide Antagonist of IGFBP1, (i,i+7) Covalently Restrained Analog''' | '''Peptide Antagonist of IGFBP1, (i,i+7) Covalently Restrained Analog''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IN2 OCA]. | |
==Reference== | ==Reference== | ||
Structure-function analysis of a phage display-derived peptide that binds to insulin-like growth factor binding protein 1., Skelton NJ, Chen YM, Dubree N, Quan C, Jackson DY, Cochran A, Zobel K, Deshayes K, Baca M, Pisabarro MT, Lowman HB, Biochemistry. 2001 Jul 24;40(29):8487-98. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11456486 11456486] | Structure-function analysis of a phage display-derived peptide that binds to insulin-like growth factor binding protein 1., Skelton NJ, Chen YM, Dubree N, Quan C, Jackson DY, Cochran A, Zobel K, Deshayes K, Baca M, Pisabarro MT, Lowman HB, Biochemistry. 2001 Jul 24;40(29):8487-98. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11456486 11456486] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Baca, M.]] | [[Category: Baca, M.]] | ||
[[Category: Chen, Y M.]] | [[Category: Chen, Y M.]] | ||
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[[Category: Skelton, N J.]] | [[Category: Skelton, N J.]] | ||
[[Category: Zobel, K.]] | [[Category: Zobel, K.]] | ||
| - | [[Category: | + | [[Category: Covalently constrained helix]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:10:32 2008'' | |
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Revision as of 17:10, 2 May 2008
Peptide Antagonist of IGFBP1, (i,i+7) Covalently Restrained Analog
Overview
Highly structured, peptide antagonists of the interaction between insulin-like growth factor 1 (IGF-I) and IGF binding protein 1 (IGFBP-1) have recently been discovered by phage display of naive peptide libraries [Lowman, H. B., et al. (1998) Biochemistry 37, 8870--8878]. We now report a detailed analysis of the features of this turn-helix peptide motif that are necessary for IGFBP-1 binding and structural integrity. Further rounds of phage randomization indicate the importance of residues contributing to a hydrophobic patch on one face of the helix. Alanine-scanning substitutions confirm that the hydrophobic residues are necessary for binding. However, structural analysis by NMR spectroscopy indicates that some of these analogues are less well folded. Structured, high-affinity analogues that lack the disulfide bond were prepared by introducing a covalent constraint between side chains at positions i and i + 7 or i + 8 within the helix. Analogues based on this scaffold demonstrate that a helical conformation is present in the bound state, and that hydrophobic side chains in this helix, and residues immediately preceding it, interact with IGFBP-1. By comparison of alanine scanning data for IGF-I and the turn-helix peptide, we propose a model for common surface features of these molecules that recognize IGFBP-1.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structure-function analysis of a phage display-derived peptide that binds to insulin-like growth factor binding protein 1., Skelton NJ, Chen YM, Dubree N, Quan C, Jackson DY, Cochran A, Zobel K, Deshayes K, Baca M, Pisabarro MT, Lowman HB, Biochemistry. 2001 Jul 24;40(29):8487-98. PMID:11456486 Page seeded by OCA on Fri May 2 20:10:32 2008
