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| | ==Crystal Structure of SusF from Bacteroides thetaiotaomicron== | | ==Crystal Structure of SusF from Bacteroides thetaiotaomicron== |
| - | <StructureSection load='4fe9' size='340' side='right' caption='[[4fe9]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='4fe9' size='340' side='right'caption='[[4fe9]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fe9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bactn Bactn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FE9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fe9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron_VPI-5482 Bacteroides thetaiotaomicron VPI-5482]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FE9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MTT:MALTOTETRAOSE'>MTT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PRD_900010:alpha-maltotetraose'>PRD_900010</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fch|4fch]], [[4fem|4fem]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fe9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fe9 OCA], [https://pdbe.org/4fe9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fe9 RCSB], [https://www.ebi.ac.uk/pdbsum/4fe9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fe9 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BT_3699, susF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226186 BACTN])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fe9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fe9 OCA], [http://pdbe.org/4fe9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fe9 RCSB], [http://www.ebi.ac.uk/pdbsum/4fe9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fe9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SUSF_BACTN SUSF_BACTN]] Starch-binding protein present at the surface of the cell. Mediates starch-binding before starch transport in the periplasm for degradation. SusE and SusF do not constitute the major starch-binding proteins in starch degradation pathway. Has lower affinity for starch compared to SusE.<ref>PMID:10986238</ref> <ref>PMID:11717282</ref> <ref>PMID:22910908</ref> | + | [https://www.uniprot.org/uniprot/SUSF_BACTN SUSF_BACTN] Starch-binding protein present at the surface of the cell. Mediates starch-binding before starch transport in the periplasm for degradation. SusE and SusF do not constitute the major starch-binding proteins in starch degradation pathway. Has lower affinity for starch compared to SusE.<ref>PMID:10986238</ref> <ref>PMID:11717282</ref> <ref>PMID:22910908</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bactn]] | + | [[Category: Bacteroides thetaiotaomicron VPI-5482]] |
| - | [[Category: Cameron, E A]]
| + | [[Category: Large Structures]] |
| - | [[Category: Koropatkin, N M]]
| + | [[Category: Cameron EA]] |
| - | [[Category: Martens, E C]]
| + | [[Category: Koropatkin NM]] |
| - | [[Category: Carbohydrate-binding protein]]
| + | [[Category: Martens EC]] |
| - | [[Category: Extracellular surface]] | + | |
| - | [[Category: Ig fold]] | + | |
| - | [[Category: Outermembrane]] | + | |
| - | [[Category: Starch binding]] | + | |
| Structural highlights
4fe9 is a 1 chain structure with sequence from Bacteroides thetaiotaomicron VPI-5482. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
SUSF_BACTN Starch-binding protein present at the surface of the cell. Mediates starch-binding before starch transport in the periplasm for degradation. SusE and SusF do not constitute the major starch-binding proteins in starch degradation pathway. Has lower affinity for starch compared to SusE.[1] [2] [3]
Publication Abstract from PubMed
Human colonic bacteria are necessary for the digestion of many dietary polysaccharides. The intestinal symbiont Bacteroides thetaiotaomicron uses five outer membrane proteins to bind and degrade starch. Here, we report the x-ray crystallographic structures of SusE and SusF, two outer membrane proteins composed of tandem starch specific carbohydrate-binding modules (CBMs) with no enzymatic activity. Examination of the two CBMs in SusE and three CBMs in SusF reveals subtle differences in the way each binds starch and is reflected in their Kds for both high molecular weight starch and small maltooligosaccharides. Thus, each site seems to have a unique starch preference that may enable these proteins to interact with different regions of starch or its breakdown products. Proteins similar to SusE and SusF are encoded in many other polysaccharide utilization loci that are possessed by human gut bacteria in the phylum Bacteroidetes. Thus, these proteins are likely to play an important role in carbohydrate metabolism in these abundant symbiotic species. Understanding structural changes that diversify and adapt related proteins in the human gut microbial community will be critical to understanding the detailed mechanistic roles that they perform in the complex digestive ecosystem.
Multi-domain carbohydrate-binding proteins involved in Bacteroides thetaiotaomicron starch metabolism.,Cameron EA, Maynard MA, Smith CJ, Smith TJ, Koropatkin NM, Martens EC J Biol Chem. 2012 Aug 21. PMID:22910908[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Shipman JA, Berleman JE, Salyers AA. Characterization of four outer membrane proteins involved in binding starch to the cell surface of Bacteroides thetaiotaomicron. J Bacteriol. 2000 Oct;182(19):5365-72. PMID:10986238
- ↑ Cho KH, Salyers AA. Biochemical analysis of interactions between outer membrane proteins that contribute to starch utilization by Bacteroides thetaiotaomicron. J Bacteriol. 2001 Dec;183(24):7224-30. PMID:11717282
- ↑ Cameron EA, Maynard MA, Smith CJ, Smith TJ, Koropatkin NM, Martens EC. Multi-domain carbohydrate-binding proteins involved in Bacteroides thetaiotaomicron starch metabolism. J Biol Chem. 2012 Aug 21. PMID:22910908 doi:http://dx.doi.org/10.1074/jbc.M112.397380
- ↑ Cameron EA, Maynard MA, Smith CJ, Smith TJ, Koropatkin NM, Martens EC. Multi-domain carbohydrate-binding proteins involved in Bacteroides thetaiotaomicron starch metabolism. J Biol Chem. 2012 Aug 21. PMID:22910908 doi:http://dx.doi.org/10.1074/jbc.M112.397380
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