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From Proteopedia
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== UvrD == | == UvrD == | ||
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<scene name='92/925553/Uvrd/1'>UvrD</scene>, also known as Helicase II, is one of many components responsible in repairing DNA damage. UvrD is involved in nucleotide excision repair (NER) and transcription-coupled DNA repair (TCR repair). TCR repair occurs during transcription when Helicase II binds to a RNA polymerase (RNAP) and slides backwards in order to fix the mismatched nucleotide. One of the main structural components of how UvrD binds to RNAP is by having a Tudor-domain like fold. This helps better explain the UvrD-RNAP interaction, which is a better explanation than just having nucleic-acid affinity. A main cause in damage to DNA, which requires for nucleotide excision repair is from UV radiation, which can come from multiple sources, including sunlight and UV light from tanning beds. | <scene name='92/925553/Uvrd/1'>UvrD</scene>, also known as Helicase II, is one of many components responsible in repairing DNA damage. UvrD is involved in nucleotide excision repair (NER) and transcription-coupled DNA repair (TCR repair). TCR repair occurs during transcription when Helicase II binds to a RNA polymerase (RNAP) and slides backwards in order to fix the mismatched nucleotide. One of the main structural components of how UvrD binds to RNAP is by having a Tudor-domain like fold. This helps better explain the UvrD-RNAP interaction, which is a better explanation than just having nucleic-acid affinity. A main cause in damage to DNA, which requires for nucleotide excision repair is from UV radiation, which can come from multiple sources, including sunlight and UV light from tanning beds. | ||
Helicases use energy from nucleoside triphosphate hydrolysis to unwind double helices in metabolic pathways using nucleic acids. A nucleoside triphosphate (NTP) is a nucleotide with a nitrogenous base bound to a 5-carbon sugar with 3 phosphates attached. Phosphates are typically used to store energy that is released when breaking bonds to drive catabolic reactions. | Helicases use energy from nucleoside triphosphate hydrolysis to unwind double helices in metabolic pathways using nucleic acids. A nucleoside triphosphate (NTP) is a nucleotide with a nitrogenous base bound to a 5-carbon sugar with 3 phosphates attached. Phosphates are typically used to store energy that is released when breaking bonds to drive catabolic reactions. | ||
Revision as of 01:04, 10 October 2022
Contents |
DNA RECOGNITION BY GAL4: STRUCTURE OF A PROTEIN/DNA COMPLEX
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UvrD
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, also known as Helicase II, is one of many components responsible in repairing DNA damage. UvrD is involved in nucleotide excision repair (NER) and transcription-coupled DNA repair (TCR repair). TCR repair occurs during transcription when Helicase II binds to a RNA polymerase (RNAP) and slides backwards in order to fix the mismatched nucleotide. One of the main structural components of how UvrD binds to RNAP is by having a Tudor-domain like fold. This helps better explain the UvrD-RNAP interaction, which is a better explanation than just having nucleic-acid affinity. A main cause in damage to DNA, which requires for nucleotide excision repair is from UV radiation, which can come from multiple sources, including sunlight and UV light from tanning beds. Helicases use energy from nucleoside triphosphate hydrolysis to unwind double helices in metabolic pathways using nucleic acids. A nucleoside triphosphate (NTP) is a nucleotide with a nitrogenous base bound to a 5-carbon sugar with 3 phosphates attached. Phosphates are typically used to store energy that is released when breaking bonds to drive catabolic reactions. Helicases are also involved in multiple processes, including RNAs and DNAs, single stranded and double stranded. Helicases were found in the 1970’s to be DNA-dependent ATPases, meaning that they use ATP hydrolysis to complete its interactions with the different types of nucleic acids it comes into contact with. There are multiple types of helicases, with some being for RNA and DNA. Helicase II, also called UvrD is the founding member of SF1, one group of six superfamiliies used to identify helicases. SF1 and SF2 members share seven conserved sequence motifs that are involved in NTP binding. These are located between the two RecA-like domains in other group members of SF1 and SF2. UvrD is important in replication, recombination, and repair from ultraviolet damage and mismatched base pairs. Go into NER from the book..
There are 7 sequence motifs and a Q motif conserved among the SF1 and the SF2. I, Ia, II-VI) are involved in ATP binding. Motifs Ia, III, and V are also involved in ssDNA binding. Motif IV is reported to be unique in SF1. They found in their paper, seven new sequence motifs conserved among UvrD homologs. They are Ib, Ic, Id, IVb, IVc, Va, and VIa. These conserved residues are involved in DNA binding or domain 1B and 2B interactions.
UvrD Binding Site for DNA
UvrD Binding Site with RNAP and nontemplate strand
UvrD Tudor-Domain
What is a Tudor Domain? It is a methyl-lysine and methyl arginine binding domain present in proteins.
