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| | ==Crystal Structure of HVEM== | | ==Crystal Structure of HVEM== |
| - | <StructureSection load='4fhq' size='340' side='right' caption='[[4fhq]], [[Resolution|resolution]] 2.25Å' scene=''> | + | <StructureSection load='4fhq' size='340' side='right'caption='[[4fhq]], [[Resolution|resolution]] 2.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fhq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FHQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FHQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fhq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FHQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FHQ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2aw2|2aw2]], [[1jma|1jma]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fhq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fhq OCA], [https://pdbe.org/4fhq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fhq RCSB], [https://www.ebi.ac.uk/pdbsum/4fhq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fhq ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HVEA, HVEM, TNFRSF14, UNQ329/PRO509 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fhq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fhq OCA], [http://pdbe.org/4fhq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fhq RCSB], [http://www.ebi.ac.uk/pdbsum/4fhq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fhq ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TNR14_HUMAN TNR14_HUMAN]] Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.<ref>PMID:8898196</ref> | + | [https://www.uniprot.org/uniprot/TNR14_HUMAN TNR14_HUMAN] Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.<ref>PMID:8898196</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Tumor necrosis factor receptor|Tumor necrosis factor receptor]] | + | *[[Tumor necrosis factor receptor 3D structures|Tumor necrosis factor receptor 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Almo, S C]] | + | [[Category: Large Structures]] |
| - | [[Category: Bhosle, R C]] | + | [[Category: Almo SC]] |
| - | [[Category: IFN, Atoms-to-Animals:.The Immune Function Network]] | + | [[Category: Bhosle RC]] |
| - | [[Category: Liu, W]] | + | [[Category: Liu W]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Nathenson SG]] |
| - | [[Category: Nathenson, S G]] | + | [[Category: Patskovsky Y]] |
| - | [[Category: Patskovsky, Y]] | + | [[Category: Zhan C]] |
| - | [[Category: Zhan, C]] | + | |
| - | [[Category: Atoms-to-animals: the immune function network]]
| + | |
| - | [[Category: Btla]]
| + | |
| - | [[Category: Cd160]]
| + | |
| - | [[Category: Cysteine rich domain]]
| + | |
| - | [[Category: Gd of hsv]]
| + | |
| - | [[Category: Ifn]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Receptor]]
| + | |
| - | [[Category: Tnf receptor]]
| + | |
| - | [[Category: Tnf14]]
| + | |
| - | [[Category: Tnfrsf]]
| + | |
| Structural highlights
Function
TNR14_HUMAN Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.[1]
Publication Abstract from PubMed
Many immune ligands and receptors are potential drug targets, which delicately manipulate a wide range of immune responses. We describe here the successful application of an efficient method to dramatically improve the heterologous expression levels in Drosophila Schneider 2 cells, which enables the high-throughput production of several important immune ligands/receptors for raising antibodies, and for the structural and functional analyses. As an example, we purified the protein and characterized the structure of the immune receptor herpesvirus entry mediator (HVEM, TNFRSF14). HVEM is a member of tumor necrosis factor receptor superfamily, which is recognized by herpes simplex virus glycoprotein D (gD) and facilitates viral entry. HVEM participates in a range of interactions with other cell surface molecules, including LIGHT, BTLA, and CD160 to modulate a wide range of immune processes in CD4+ and CD8+ T cells, as well as NK cells. Due to the involvement of HVEM in these diverse signaling interactions, crystal structures of HVEM in complex with gD or BTLA have been previously reported. Here, we report the structure of HVEM in the absence of any ligands.
Increased Heterologous Protein Expression in Drosophila S2 Cells for Massive Production of Immune Ligands/Receptors and Structural Analysis of Human HVEM.,Liu W, Vigdorovich V, Zhan C, Patskovsky Y, Bonanno JB, Nathenson SG, Almo SC Mol Biotechnol. 2015 Jul 23. PMID:26202493[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Montgomery RI, Warner MS, Lum BJ, Spear PG. Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family. Cell. 1996 Nov 1;87(3):427-36. PMID:8898196
- ↑ Liu W, Vigdorovich V, Zhan C, Patskovsky Y, Bonanno JB, Nathenson SG, Almo SC. Increased Heterologous Protein Expression in Drosophila S2 Cells for Massive Production of Immune Ligands/Receptors and Structural Analysis of Human HVEM. Mol Biotechnol. 2015 Jul 23. PMID:26202493 doi:http://dx.doi.org/10.1007/s12033-015-9881-2
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