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| | <StructureSection load='4fm4' size='340' side='right'caption='[[4fm4]], [[Resolution|resolution]] 2.38Å' scene=''> | | <StructureSection load='4fm4' size='340' side='right'caption='[[4fm4]], [[Resolution|resolution]] 2.38Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fm4]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11996 Atcc 11996]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FM4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fm4]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FM4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FM4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fm4 OCA], [https://pdbe.org/4fm4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fm4 RCSB], [https://www.ebi.ac.uk/pdbsum/4fm4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fm4 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fm4 OCA], [http://pdbe.org/4fm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fm4 RCSB], [http://www.ebi.ac.uk/pdbsum/4fm4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fm4 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/J9PBS0_COMTE J9PBS0_COMTE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Journal:JBIC:32|Journal:JBIC:32]] | |
| | *[[Nitrile hydratase|Nitrile hydratase]] | | *[[Nitrile hydratase|Nitrile hydratase]] |
| | == References == | | == References == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 11996]] | + | [[Category: Comamonas testosteroni]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Nitrile hydratase]]
| + | [[Category: Bornscheuer U]] |
| - | [[Category: Bornscheuer, U]] | + | [[Category: Gumataotao N]] |
| - | [[Category: Gumataotao, N]] | + | [[Category: Holz RC]] |
| - | [[Category: Holz, R C]] | + | [[Category: Kuhn ML]] |
| - | [[Category: Kuhn, M L]] | + | [[Category: Liu D]] |
| - | [[Category: Liu, D]] | + | [[Category: Martinez S]] |
| - | [[Category: Martinez, S]] | + | |
| - | [[Category: Hydrolysis]]
| + | |
| - | [[Category: Iron type hydratase]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Sulfinic acid]]
| + | |
| Structural highlights
Function
J9PBS0_COMTE
Publication Abstract from PubMed
We report herein the functional expression of an Fe-type nitrile hydratase (NHase) without the co-expression of an activator protein or the Escherichia coli chaperone proteins GroES/EL. Soluble protein was obtained when the alpha- and beta-subunit genes of the Fe-type NHase Comamonas testosteroni Ni1 (CtNHase) were synthesized with optimized E. coli codon usage and co-expressed. As a control, the Fe-type NHase from Rhodococcus equi TG328-2 (ReNHase) was expressed with (ReNHase(+Act)) and without (ReNHase(-Act)) its activator protein, establishing that expression of a fully functional, metallated ReNHase enzyme requires the co-expression of its activator protein, similar to all other Fe-type NHase enzymes reported to date, whereas the CtNHase does not. The X-ray crystal structure of CtNHase was determined to 2.4A resolution revealing an alphabeta heterodimer, similar to other Fe-type NHase enzymes, except for two important differences. First, two His residues reside in the CtNHase active site that are not observed in other Fe-type NHase enzymes and second, the active site Fe(III) ion resides at the bottom of a wide solvent exposed channel. The solvent exposed active site, along with the two active site histidine residues, are hypothesized to play a role in iron incorporation in the absence of an activator protein.
The Fe-type nitrile hydratase from Comamonas testosteroni Ni1 does not require an activator accessory protein for expression in Escherichia coli.,Kuhn ML, Martinez S, Gumataotao N, Bornscheuer U, Liu D, Holz RC Biochem Biophys Res Commun. 2012 Aug 3;424(3):365-70. Epub 2012 Jun 16. PMID:22713452[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kuhn ML, Martinez S, Gumataotao N, Bornscheuer U, Liu D, Holz RC. The Fe-type nitrile hydratase from Comamonas testosteroni Ni1 does not require an activator accessory protein for expression in Escherichia coli. Biochem Biophys Res Commun. 2012 Aug 3;424(3):365-70. Epub 2012 Jun 16. PMID:22713452 doi:10.1016/j.bbrc.2012.06.036
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