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| | ==SPFH domain of mouse stomatin (Crystal form 1)== | | ==SPFH domain of mouse stomatin (Crystal form 1)== |
| - | <StructureSection load='4fvf' size='340' side='right' caption='[[4fvf]], [[Resolution|resolution]] 2.46Å' scene=''> | + | <StructureSection load='4fvf' size='340' side='right'caption='[[4fvf]], [[Resolution|resolution]] 2.46Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fvf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FVF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fvf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FVF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fvj|4fvj]], [[4fvg|4fvg]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fvf OCA], [https://pdbe.org/4fvf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fvf RCSB], [https://www.ebi.ac.uk/pdbsum/4fvf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fvf ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Epb7.2, Epb72, Stom ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fvf OCA], [http://pdbe.org/4fvf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fvf RCSB], [http://www.ebi.ac.uk/pdbsum/4fvf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fvf ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/STOM_MOUSE STOM_MOUSE]] Regulates ion channel activity and transmembrane ion transport. Regulates ASIC2 and ASIC3 channel activity.<ref>PMID:15471860</ref> <ref>PMID:22850675</ref> | + | [https://www.uniprot.org/uniprot/STOM_MOUSE STOM_MOUSE] Regulates ion channel activity and transmembrane ion transport. Regulates ASIC2 and ASIC3 channel activity.<ref>PMID:15471860</ref> <ref>PMID:22850675</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Brand, J]] | + | [[Category: Mus musculus]] |
| - | [[Category: Daumke, O]] | + | [[Category: Brand J]] |
| - | [[Category: Schwefel, D]] | + | [[Category: Daumke O]] |
| - | [[Category: Membrane protein]] | + | [[Category: Schwefel D]] |
| - | [[Category: Mixed alpha-beta]]
| + | |
| - | [[Category: Scaffolding protein]]
| + | |
| Structural highlights
Function
STOM_MOUSE Regulates ion channel activity and transmembrane ion transport. Regulates ASIC2 and ASIC3 channel activity.[1] [2]
Publication Abstract from PubMed
Stomatin proteins oligomerize at membranes and have been implicated in ion channel regulation and membrane trafficking. To obtain mechanistic insights into their function, we determined three crystal structures of the conserved stomatin domain of mouse stomatin that assembles into a banana-shaped dimer. We show that dimerization is crucial for the repression of acid-sensing ion channel 3 (ASIC3) activity. A hydrophobic pocket at the inside of the concave surface is open in the presence of an internal peptide ligand and closes in the absence of this ligand, and we demonstrate a function of this pocket in the inhibition of ASIC3 activity. In one crystal form, stomatin assembles via two conserved surfaces into a cylindrical oligomer, and these oligomerization surfaces are also essential for the inhibition of ASIC3-mediated currents. The assembly mode of stomatin uncovered in this study might serve as a model to understand oligomerization processes of related membrane-remodelling proteins, such as flotillin and prohibitin.
A stomatin dimer modulates the activity of acid-sensing ion channels.,Brand J, Smith ES, Schwefel D, Lapatsina L, Poole K, Omerbasic D, Kozlenkov A, Behlke J, Lewin GR, Daumke O EMBO J. 2012 Jul 31. doi: 10.1038/emboj.2012.203. PMID:22850675[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Price MP, Thompson RJ, Eshcol JO, Wemmie JA, Benson CJ. Stomatin modulates gating of acid-sensing ion channels. J Biol Chem. 2004 Dec 17;279(51):53886-91. Epub 2004 Oct 7. PMID:15471860 doi:http://dx.doi.org/10.1074/jbc.M407708200
- ↑ Brand J, Smith ES, Schwefel D, Lapatsina L, Poole K, Omerbasic D, Kozlenkov A, Behlke J, Lewin GR, Daumke O. A stomatin dimer modulates the activity of acid-sensing ion channels. EMBO J. 2012 Jul 31. doi: 10.1038/emboj.2012.203. PMID:22850675 doi:10.1038/emboj.2012.203
- ↑ Brand J, Smith ES, Schwefel D, Lapatsina L, Poole K, Omerbasic D, Kozlenkov A, Behlke J, Lewin GR, Daumke O. A stomatin dimer modulates the activity of acid-sensing ion channels. EMBO J. 2012 Jul 31. doi: 10.1038/emboj.2012.203. PMID:22850675 doi:10.1038/emboj.2012.203
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