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| | ==Crystal structure of MST4-MO25 complex with WSF motif== | | ==Crystal structure of MST4-MO25 complex with WSF motif== |
| - | <StructureSection load='4fzd' size='340' side='right' caption='[[4fzd]], [[Resolution|resolution]] 3.25Å' scene=''> | + | <StructureSection load='4fzd' size='340' side='right'caption='[[4fzd]], [[Resolution|resolution]] 3.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fzd]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FZD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fzd]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FZD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fza|4fza]], [[4fzf|4fzf]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzd OCA], [https://pdbe.org/4fzd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fzd RCSB], [https://www.ebi.ac.uk/pdbsum/4fzd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fzd ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CAB39, MO25, CGI-66 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), MST4, MASK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzd OCA], [http://pdbe.org/4fzd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fzd RCSB], [http://www.ebi.ac.uk/pdbsum/4fzd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fzd ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CAB39_HUMAN CAB39_HUMAN]] Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1. [[http://www.uniprot.org/uniprot/MST4_HUMAN MST4_HUMAN]] Mediator of cell growth. Modulates apoptosis.<ref>PMID:17360971</ref> | + | [https://www.uniprot.org/uniprot/CAB39_HUMAN CAB39_HUMAN] Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Serine/threonine protein kinase|Serine/threonine protein kinase]] | + | *[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Non-specific serine/threonine protein kinase]] | + | [[Category: Large Structures]] |
| - | [[Category: Shi, Z B]] | + | [[Category: Shi ZB]] |
| - | [[Category: Zhou, Z C]] | + | [[Category: Zhou ZC]] |
| - | [[Category: Atp binding]]
| + | |
| - | [[Category: Protein ser/thr kinase]]
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| - | [[Category: Scaffold protein]]
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| - | [[Category: Signaling protein-transferase complex]]
| + | |
| Structural highlights
Function
CAB39_HUMAN Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1.
Publication Abstract from PubMed
Mammalian STE20-like kinase MST4 regulates multiple cellular aspects such as cell polarity and proliferation. MST4 acts downstream of LKB1/MO25/STRAD complex to induce brush border formation. MO25 directly interacts with MST4 to promote its kinase activity. Here, we report the crystal structure of MST4 in complex with MO25. Association of MO25 rotates the alphaC helix of MST4 toward its catalytic core, stabilizing the alphaC helix in an active position. The kinase domain of MST4 forms a specific homodimer that is required for trans-autophosphorylation. MO25-stimulated activation of MST4 promotes apoptosis in HEK293T cells. Atomic resolution permitted the study of interface mutations capable of disrupting the MST4-MO25 interaction or the kinase-domain-mediated homodimerization. These mutations impaired MST4 kinase activation and function within the cell. Collectively, our study identifies the activation mechanism of MST4 and provides a structural basis for further functional study.
Structure of the MST4 in Complex with MO25 Provides Insights into Its Activation Mechanism.,Shi Z, Jiao S, Zhang Z, Ma M, Zhang Z, Chen C, Wang K, Wang H, Wang W, Zhang L, Zhao Y, Zhou Z Structure. 2013 Feb 19. pii: S0969-2126(13)00014-2. doi:, 10.1016/j.str.2013.01.007. PMID:23434407[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Shi Z, Jiao S, Zhang Z, Ma M, Zhang Z, Chen C, Wang K, Wang H, Wang W, Zhang L, Zhao Y, Zhou Z. Structure of the MST4 in Complex with MO25 Provides Insights into Its Activation Mechanism. Structure. 2013 Feb 19. pii: S0969-2126(13)00014-2. doi:, 10.1016/j.str.2013.01.007. PMID:23434407 doi:http://dx.doi.org/10.1016/j.str.2013.01.007
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