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| | ==Crystal Structure of SsfS6, Streptomyces sp. SF2575 glycosyltransferase== | | ==Crystal Structure of SsfS6, Streptomyces sp. SF2575 glycosyltransferase== |
| - | <StructureSection load='4fzr' size='340' side='right' caption='[[4fzr]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='4fzr' size='340' side='right'caption='[[4fzr]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fzr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._sf2575 Streptomyces sp. sf2575]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FZR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fzr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._SF2575 Streptomyces sp. SF2575]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FZR FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4g2t|4g2t]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzr OCA], [https://pdbe.org/4fzr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fzr RCSB], [https://www.ebi.ac.uk/pdbsum/4fzr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fzr ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ssfS6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746675 Streptomyces sp. SF2575])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzr OCA], [http://pdbe.org/4fzr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fzr RCSB], [http://www.ebi.ac.uk/pdbsum/4fzr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fzr ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D6MSX4_9ACTN D6MSX4_9ACTN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Streptomyces sp. sf2575]] | + | [[Category: Large Structures]] |
| - | [[Category: Bingman, C A]] | + | [[Category: Streptomyces sp. SF2575]] |
| - | [[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]] | + | [[Category: Bingman CA]] |
| - | [[Category: Phillips, G N]] | + | [[Category: Phillips Jr GN]] |
| - | [[Category: Singh, S]] | + | [[Category: Singh S]] |
| - | [[Category: Thorson, J S]] | + | [[Category: Thorson JS]] |
| - | [[Category: Wang, F]] | + | [[Category: Wang F]] |
| - | [[Category: Zhou, M]] | + | [[Category: Zhou M]] |
| - | [[Category: Enzyme discovery for natural product biosynthesis]]
| + | |
| - | [[Category: Glycosyltransferase]]
| + | |
| - | [[Category: Natpro]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Psi-biology]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Transferase]]
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| Structural highlights
Function
D6MSX4_9ACTN
Publication Abstract from PubMed
The molecule known as SF2575 from Streptomyces sp. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 in vivo. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial C-glycosyltransferase (C-GT) that forms the C-C glycosidic bond between the sugar and the SF2575 tetracycline-like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 A resolution. The structures reveal SsfS6 to adopt a GT-B fold wherein the TDP and docked putative aglycon are consistent with the overall C-glycosylation reaction. As one of only a few existing structures for C-glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer C-glycosylation.Proteins 2013. (c) 2013 Wiley Periodicals, Inc.
Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis.,Wang F, Zhou M, Singh S, Yennamalli RM, Bingman CA, Thorson JS, Phillips GN Jr Proteins. 2013 Mar 22. doi: 10.1002/prot.24289. PMID:23526584[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wang F, Zhou M, Singh S, Yennamalli RM, Bingman CA, Thorson JS, Phillips GN Jr. Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis. Proteins. 2013 Mar 22. doi: 10.1002/prot.24289. PMID:23526584 doi:10.1002/prot.24289
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