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| | ==Crystal structure of a Salmonella type III secretion system protein== | | ==Crystal structure of a Salmonella type III secretion system protein== |
| - | <StructureSection load='4g2s' size='340' side='right' caption='[[4g2s]], [[Resolution|resolution]] 1.86Å' scene=''> | + | <StructureSection load='4g2s' size='340' side='right'caption='[[4g2s]], [[Resolution|resolution]] 1.86Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4g2s]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G2S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G2S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4g2s]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G2S FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4g08|4g08]], [[4g1i|4g1i]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g2s OCA], [https://pdbe.org/4g2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g2s RCSB], [https://www.ebi.ac.uk/pdbsum/4g2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g2s ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">prgH, STM2874 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g2s OCA], [http://pdbe.org/4g2s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g2s RCSB], [http://www.ebi.ac.uk/pdbsum/4g2s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g2s ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PRGH_SALTY PRGH_SALTY]] Required for invasion of epithelial cells. | + | [https://www.uniprot.org/uniprot/PRGH_SALTY PRGH_SALTY] Required for invasion of epithelial cells. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus typhimurium loeffler 1892]] | + | [[Category: Large Structures]] |
| - | [[Category: Strynadka, N C.J]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium]] |
| - | [[Category: Vuckovic, M]] | + | [[Category: Strynadka NCJ]] |
| - | [[Category: Worrall, L J]] | + | [[Category: Vuckovic M]] |
| - | [[Category: Cell invasion]] | + | [[Category: Worrall LJ]] |
| - | [[Category: Fha domain]]
| + | |
| Structural highlights
Function
PRGH_SALTY Required for invasion of epithelial cells.
Publication Abstract from PubMed
The T3SS injectisome is a syringe-shaped macromolecular assembly found in pathogenic Gram-negative bacteria that allows for the direct delivery of virulence effectors into host cells. It is composed of a "basal body", a lock-nut structure spanning both bacterial membranes, and a "needle" that protrudes away from the bacterial surface. A hollow channel spans throughout the apparatus, permitting the translocation of effector proteins from the bacterial cytosol to the host plasma membrane. The basal body is composed largely of three membrane-embedded proteins that form oligomerized concentric rings. Here, we report the crystal structures of three domains of the prototypical Salmonella SPI-1 basal body, and use a new approach incorporating symmetric flexible backbone docking and EM data to produce a model for their oligomeric assembly. The obtained models, validated by biochemical and in vivo assays, reveal the molecular details of the interactions driving basal body assembly, and notably demonstrate a conserved oligomerization mechanism.
A Refined Model of the Prototypical Salmonella SPI-1 T3SS Basal Body Reveals the Molecular Basis for Its Assembly.,Bergeron JR, Worrall LJ, Sgourakis NG, Dimaio F, Pfuetzner RA, Felise HB, Vuckovic M, Yu AC, Miller SI, Baker D, Strynadka NC PLoS Pathog. 2013 Apr;9(4):e1003307. doi: 10.1371/journal.ppat.1003307. Epub 2013, Apr 25. PMID:23633951[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bergeron JR, Worrall LJ, Sgourakis NG, Dimaio F, Pfuetzner RA, Felise HB, Vuckovic M, Yu AC, Miller SI, Baker D, Strynadka NC. A Refined Model of the Prototypical Salmonella SPI-1 T3SS Basal Body Reveals the Molecular Basis for Its Assembly. PLoS Pathog. 2013 Apr;9(4):e1003307. doi: 10.1371/journal.ppat.1003307. Epub 2013, Apr 25. PMID:23633951 doi:10.1371/journal.ppat.1003307
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