|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of the murine cytomegalovirus MHC-I homolog m152 with ligand RAE-1 gamma== | | ==Crystal structure of the murine cytomegalovirus MHC-I homolog m152 with ligand RAE-1 gamma== |
| - | <StructureSection load='4g59' size='340' side='right' caption='[[4g59]], [[Resolution|resolution]] 2.44Å' scene=''> | + | <StructureSection load='4g59' size='340' side='right'caption='[[4g59]], [[Resolution|resolution]] 2.44Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4g59]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Muhv1 Muhv1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G59 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4g59]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Murid_betaherpesvirus_1 Murid betaherpesvirus 1] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G59 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Raet1c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), m152 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10366 MUHV1])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g59 OCA], [https://pdbe.org/4g59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g59 RCSB], [https://www.ebi.ac.uk/pdbsum/4g59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g59 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g59 OCA], [http://pdbe.org/4g59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g59 RCSB], [http://www.ebi.ac.uk/pdbsum/4g59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g59 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RAE1C_MOUSE RAE1C_MOUSE]] Acts as a ligand for NKG2D.<ref>PMID:10894171</ref> | + | [https://www.uniprot.org/uniprot/RAE1C_MOUSE RAE1C_MOUSE] Acts as a ligand for NKG2D.<ref>PMID:10894171</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Muhv1]] | + | [[Category: Murid betaherpesvirus 1]] |
| - | [[Category: Margulies, D H]] | + | [[Category: Mus musculus]] |
| - | [[Category: Natarajan, K]] | + | [[Category: Margulies DH]] |
| - | [[Category: Wang, R]] | + | [[Category: Natarajan K]] |
| - | [[Category: Immune system]] | + | [[Category: Wang R]] |
| - | [[Category: Immunoevasion]]
| + | |
| - | [[Category: Mhc-i fold]]
| + | |
| - | [[Category: Stress induced ligand]]
| + | |
| Structural highlights
Function
RAE1C_MOUSE Acts as a ligand for NKG2D.[1]
Publication Abstract from PubMed
Natural killer (NK) cells are activated by engagement of the NKG2D receptor with ligands on target cells stressed by infection or tumorigenesis. Several human and rodent cytomegalovirus (CMV) immunoevasins down-regulate surface expression of NKG2D ligands. The mouse CMV MHC class I (MHC-I)-like m152/gp40 glycoprotein down-regulates retinoic acid early inducible-1 (RAE1) NKG2D ligands as well as host MHC-I. Here we describe the crystal structure of an m152/RAE1gamma complex and confirm the intermolecular contacts by mutagenesis. m152 interacts in a pincer-like manner with two sites on the alpha1 and alpha2 helices of RAE1 reminiscent of the NKG2D interaction with RAE1. This structure of an MHC-I-like immunoevasin/MHC-I-like ligand complex explains the binding specificity of m152 for RAE1 and allows modeling of the interaction of m152 with classical MHC-I and of related viral immunoevasins.
Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1gamma reveals a paradigm for MHC/MHC interaction in immune evasion.,Wang R, Natarajan K, Revilleza MJ, Boyd LF, Zhi L, Zhao H, Robinson H, Margulies DH Proc Natl Acad Sci U S A. 2012 Nov 19. PMID:23169621[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cerwenka A, Bakker AB, McClanahan T, Wagner J, Wu J, Phillips JH, Lanier LL. Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice. Immunity. 2000 Jun;12(6):721-7. PMID:10894171
- ↑ Wang R, Natarajan K, Revilleza MJ, Boyd LF, Zhi L, Zhao H, Robinson H, Margulies DH. Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1gamma reveals a paradigm for MHC/MHC interaction in immune evasion. Proc Natl Acad Sci U S A. 2012 Nov 19. PMID:23169621 doi:10.1073/pnas.1214088109
|
