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| | ==G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex== | | ==G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex== |
| - | <StructureSection load='4g6d' size='340' side='right' caption='[[4g6d]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='4g6d' size='340' side='right'caption='[[4g6d]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4g6d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_g1 Bacteriophage g1] and [http://en.wikipedia.org/wiki/Staa8 Staa8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G6D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4g6d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325] and [https://en.wikipedia.org/wiki/Staphylococcus_virus_G1 Staphylococcus virus G1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G6D FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpoD, plaC, sigA, SAOUHSC_01662 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 STAA8])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6d OCA], [https://pdbe.org/4g6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g6d RCSB], [https://www.ebi.ac.uk/pdbsum/4g6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g6d ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6d OCA], [http://pdbe.org/4g6d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g6d RCSB], [http://www.ebi.ac.uk/pdbsum/4g6d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g6d ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SIGA_STAA8 SIGA_STAA8]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. | + | [https://www.uniprot.org/uniprot/SIGA_STAA8 SIGA_STAA8] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Sigma factor|Sigma factor]] | + | *[[Sigma factor 3D structures|Sigma factor 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacteriophage g1]] | + | [[Category: Large Structures]] |
| - | [[Category: Staa8]] | + | [[Category: Staphylococcus aureus subsp. aureus NCTC 8325]] |
| - | [[Category: Darst, S A]] | + | [[Category: Staphylococcus virus G1]] |
| - | [[Category: Osmundson, J S]] | + | [[Category: Darst SA]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Osmundson JS]] |
| - | [[Category: Staphylococcus aureus rna polymerase]]
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| Structural highlights
Function
SIGA_STAA8 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.
Publication Abstract from PubMed
Phage G1 gp67 is a 23 kDa protein that binds to the Staphylococcus aureus (Sau) RNA polymerase (RNAP) sigma(A) subunit and blocks cell growth by inhibiting transcription. We show that gp67 has little to no effect on transcription from most promoters but is a potent inhibitor of ribosomal RNA transcription. A 2.0-A-resolution crystal structure of the complex between gp67 and Sau sigma(A) domain 4 (sigma(A)(4)) explains how gp67 joins the RNAP promoter complex through sigma(A)(4) without significantly affecting sigma(A)(4) function. Our results indicate that gp67 forms a complex with RNAP at most, if not all, sigma(A)-dependent promoters, but selectively inhibits promoters that depend on an interaction between upstream DNA and the RNAP alpha-subunit C-terminal domain (alphaCTD). Thus, we reveal a promoter-specific transcription inhibition mechanism by which gp67 interacts with the RNAP promoter complex through one subunit (sigma(A)), and selectively affects the function of another subunit (alphaCTD) depending on promoter usage.
Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein.,Osmundson J, Montero-Diez C, Westblade LF, Hochschild A, Darst SA Cell. 2012 Nov 21;151(5):1005-16. doi: 10.1016/j.cell.2012.10.034. PMID:23178120[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Osmundson J, Montero-Diez C, Westblade LF, Hochschild A, Darst SA. Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein. Cell. 2012 Nov 21;151(5):1005-16. doi: 10.1016/j.cell.2012.10.034. PMID:23178120 doi:10.1016/j.cell.2012.10.034
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