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| | ==CdiA-CT/CdiI toxin and immunity complex from Burkholderia pseudomallei== | | ==CdiA-CT/CdiI toxin and immunity complex from Burkholderia pseudomallei== |
| - | <StructureSection load='4g6v' size='340' side='right' caption='[[4g6v]], [[Resolution|resolution]] 2.64Å' scene=''> | + | <StructureSection load='4g6v' size='340' side='right'caption='[[4g6v]], [[Resolution|resolution]] 2.64Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4g6v]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei_1026a Burkholderia pseudomallei 1026a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G6V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4g6v]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_1026a Burkholderia pseudomallei 1026a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G6V FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4g6u|4g6u]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6v OCA], [https://pdbe.org/4g6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g6v RCSB], [https://www.ebi.ac.uk/pdbsum/4g6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g6v ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BP1026A_3896 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1085027 Burkholderia pseudomallei 1026a]), cdiI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1085027 Burkholderia pseudomallei 1026a])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6v OCA], [http://pdbe.org/4g6v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g6v RCSB], [http://www.ebi.ac.uk/pdbsum/4g6v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g6v ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/H9T8H3_BURPE H9T8H3_BURPE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Burkholderia pseudomallei 1026a]] | | [[Category: Burkholderia pseudomallei 1026a]] |
| - | [[Category: Gerrick, E]] | + | [[Category: Large Structures]] |
| - | [[Category: Goulding, C W]] | + | [[Category: Gerrick E]] |
| - | [[Category: Hayes, C S]] | + | [[Category: Goulding CW]] |
| - | [[Category: Low, D A]] | + | [[Category: Hayes CS]] |
| - | [[Category: Morse, R P]] | + | [[Category: Low DA]] |
| - | [[Category: Nikolakakis, K]] | + | [[Category: Morse RP]] |
| - | [[Category: Willet, J]] | + | [[Category: Nikolakakis K]] |
| - | [[Category: Immunity]]
| + | [[Category: Willet J]] |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Trnase]]
| + | |
| Structural highlights
Function
H9T8H3_BURPE
Publication Abstract from PubMed
Contact-dependent growth inhibition (CDI) systems encode polymorphic toxin/immunity proteins that mediate competition between neighboring bacterial cells. We present crystal structures of CDI toxin/immunity complexes from Escherichia coli EC869 and Burkholderia pseudomallei 1026b. Despite sharing little sequence identity, the toxin domains are structurally similar and have homology to endonucleases. The EC869 toxin is a Zn(2+)-dependent DNase capable of completely degrading the genomes of target cells, whereas the Bp1026b toxin cleaves the aminoacyl acceptor stems of tRNA molecules. Each immunity protein binds and inactivates its cognate toxin in a unique manner. The EC869 toxin/immunity complex is stabilized through an unusual beta-augmentation interaction. In contrast, the Bp1026b immunity protein exploits shape and charge complementarity to occlude the toxin active site. These structures represent the initial glimpse into the CDI toxin/immunity network, illustrating how sequence-diverse toxins adopt convergent folds yet retain distinct binding interactions with cognate immunity proteins. Moreover, we present visual demonstration of CDI toxin delivery into a target cell.
Structural basis of toxicity and immunity in contact-dependent growth inhibition (CDI) systems.,Morse RP, Nikolakakis KC, Willett JL, Gerrick E, Low DA, Hayes CS, Goulding CW Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21480-5. doi:, 10.1073/pnas.1216238110. Epub 2012 Dec 10. PMID:23236156[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Morse RP, Nikolakakis KC, Willett JL, Gerrick E, Low DA, Hayes CS, Goulding CW. Structural basis of toxicity and immunity in contact-dependent growth inhibition (CDI) systems. Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21480-5. doi:, 10.1073/pnas.1216238110. Epub 2012 Dec 10. PMID:23236156 doi:http://dx.doi.org/10.1073/pnas.1216238110
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