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4gbz

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Revision as of 06:47, 26 October 2022

The structure of the MFS (major facilitator superfamily) proton:xylose symporter XylE bound to D-glucose

Structural highlights

4gbz is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

XYLE_ECOLI Uptake of D-xylose across the boundary membrane with the concomitant transport of protons into the cell (symport system).

Publication Abstract from PubMed

Glucose transporters are essential for metabolism of glucose in cells of diverse organisms from microbes to humans, exemplified by the disease-related human proteins GLUT1, 2, 3 and 4. Despite rigorous efforts, the structural information for GLUT1-4 or their homologues remains largely unknown. Here we report three related crystal structures of XylE, an Escherichia coli homologue of GLUT1-4, in complex with d-xylose, d-glucose and 6-bromo-6-deoxy-D-glucose, at resolutions of 2.8, 2.9 and 2.6 A, respectively. The structure consists of a typical major facilitator superfamily fold of 12 transmembrane segments and a unique intracellular four-helix domain. XylE was captured in an outward-facing, partly occluded conformation. Most of the important amino acids responsible for recognition of D-xylose or d-glucose are invariant in GLUT1-4, suggesting functional and mechanistic conservations. Structure-based modelling of GLUT1-4 allows mapping and interpretation of disease-related mutations. The structural and biochemical information reported here constitutes an important framework for mechanistic understanding of glucose transporters and sugar porters in general.

Crystal structure of a bacterial homologue of glucose transporters GLUT1-4.,Sun L, Zeng X, Yan C, Sun X, Gong X, Rao Y, Yan N Nature. 2012 Oct 18;490(7420):361-6. doi: 10.1038/nature11524. PMID:23075985^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sun L, Zeng X, Yan C, Sun X, Gong X, Rao Y, Yan N. Crystal structure of a bacterial homologue of glucose transporters GLUT1-4. Nature. 2012 Oct 18;490(7420):361-6. doi: 10.1038/nature11524. PMID:23075985 doi:http://dx.doi.org/10.1038/nature11524

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4gbz"

@@ Line 1: / Line 1: @@
 ==The structure of the MFS (major facilitator superfamily) proton:xylose symporter XylE bound to D-glucose==
-<StructureSection load='4gbz' size='340' side='right' caption='[[4gbz]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
+<StructureSection load='4gbz' size='340' side='right'caption='[[4gbz]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4gbz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GBZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GBZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4gbz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GBZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GBZ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gby|4gby]], [[4gc0|4gc0]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gbz OCA], [https://pdbe.org/4gbz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gbz RCSB], [https://www.ebi.ac.uk/pdbsum/4gbz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gbz ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">xylE, b4031, JW3991 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gbz OCA], [http://pdbe.org/4gbz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gbz RCSB], [http://www.ebi.ac.uk/pdbsum/4gbz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gbz ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/XYLE_ECOLI XYLE_ECOLI]] Uptake of D-xylose across the boundary membrane with the concomitant transport of protons into the cell (symport system).
+[https://www.uniprot.org/uniprot/XYLE_ECOLI XYLE_ECOLI] Uptake of D-xylose across the boundary membrane with the concomitant transport of protons into the cell (symport system).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 20: @@
 ==See Also==
-*[[Symporter|Symporter]]
+*[[Symporter 3D structures|Symporter 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
-[[Category: Sun, L F]]
+[[Category: Large Structures]]
-[[Category: Yan, C Y]]
+[[Category: Sun LF]]
-[[Category: Yan, N]]
+[[Category: Yan CY]]
-[[Category: Zeng, X]]
+[[Category: Yan N]]
-[[Category: D-xylose:proton symporter]]
+[[Category: Zeng X]]
-[[Category: Mf]]
-[[Category: Transport protein]]

4gbz

From Proteopedia

Revision as of 06:47, 26 October 2022

The structure of the MFS (major facilitator superfamily) proton:xylose symporter XylE bound to D-glucose

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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