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| | ==Malaria invasion machinery protein complex== | | ==Malaria invasion machinery protein complex== |
| - | <StructureSection load='4ggn' size='340' side='right' caption='[[4ggn]], [[Resolution|resolution]] 2.29Å' scene=''> | + | <StructureSection load='4ggn' size='340' side='right'caption='[[4ggn]], [[Resolution|resolution]] 2.29Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ggn]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Plakh Plakh] and [http://en.wikipedia.org/wiki/Playo Playo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GGN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ggn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_knowlesi_strain_H Plasmodium knowlesi strain H] and [https://en.wikipedia.org/wiki/Plasmodium_yoelii_yoelii Plasmodium yoelii yoelii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GGN FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2auc|2auc]], [[2qac|2qac]], [[4gft|4gft]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ggn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ggn OCA], [https://pdbe.org/4ggn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ggn RCSB], [https://www.ebi.ac.uk/pdbsum/4ggn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ggn ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PKH_146380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5851 PLAKH]), PY01232 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=73239 PLAYO])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ggn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ggn OCA], [http://pdbe.org/4ggn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ggn RCSB], [http://www.ebi.ac.uk/pdbsum/4ggn PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MYOA_PLAYO MYOA_PLAYO]] Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments (By similarity). | + | [https://www.uniprot.org/uniprot/A0A384KCF1_PLAKH A0A384KCF1_PLAKH] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Plakh]] | + | [[Category: Large Structures]] |
| - | [[Category: Playo]] | + | [[Category: Plasmodium knowlesi strain H]] |
| - | [[Category: Bergman, L W]] | + | [[Category: Plasmodium yoelii yoelii]] |
| - | [[Category: Hol, W G.J]] | + | [[Category: Bergman LW]] |
| - | [[Category: Khamrui, S]] | + | [[Category: Hol WGJ]] |
| - | [[Category: Turley, S]] | + | [[Category: Khamrui S]] |
| - | [[Category: Malaria invasion machinery protein]] | + | [[Category: Turley S]] |
| - | [[Category: Mtip]]
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| - | [[Category: Myoa]]
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| - | [[Category: Myoa-tail interacting protein]]
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| - | [[Category: Myosin a-tail]]
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| - | [[Category: Protein binding]]
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| Structural highlights
Function
A0A384KCF1_PLAKH
Publication Abstract from PubMed
Apicomplexan parasites enter host cells by many sophisticated steps including use of an ATP-powered invasion machinery. The machinery consists of multiple proteins, including a special myosin (MyoA) which moves along an actin fiber and which is connected to the myosin tail interaction protein (MTIP). Here we report a crystal structure of the major MyoA-binding domain (D3) of Plasmodium falciparum MTIP in complex with an anti-MTIP nanobody. In this complex, the MyoA-binding groove in MTIP-D3 is considerably less accessible than when occupied by the MyoA helix, due to a shift of two helices. The nanobody binds to an area slightly overlapping with the MyoA binding groove, covering a hydrophobic region next to the groove entrance. This provides a new avenue for arriving at compounds interfering with the invasion machinery since small molecules binding simultaneously to the nanobody binding site and the adjacent MyoA binding groove would prevent MyoA binding by MTIP.
The structure of the D3 domain of Plasmodium falciparum myosin tail interacting protein MTIP in complex with a nanobody.,Khamrui S, Turley S, Pardon E, Steyaert J, Fan E, Verlinde CL, Bergman LW, Hol WG Mol Biochem Parasitol. 2013 Aug;190(2):87-91. doi:, 10.1016/j.molbiopara.2013.06.003. Epub 2013 Jul 4. PMID:23831371[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Khamrui S, Turley S, Pardon E, Steyaert J, Fan E, Verlinde CL, Bergman LW, Hol WG. The structure of the D3 domain of Plasmodium falciparum myosin tail interacting protein MTIP in complex with a nanobody. Mol Biochem Parasitol. 2013 Aug;190(2):87-91. doi:, 10.1016/j.molbiopara.2013.06.003. Epub 2013 Jul 4. PMID:23831371 doi:http://dx.doi.org/10.1016/j.molbiopara.2013.06.003
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