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| | ==Structure and activity of AbiQ, a lactococcal anti-phage endoribonuclease belonging to the type-III toxin-antitoxin system== | | ==Structure and activity of AbiQ, a lactococcal anti-phage endoribonuclease belonging to the type-III toxin-antitoxin system== |
| - | <StructureSection load='4glk' size='340' side='right' caption='[[4glk]], [[Resolution|resolution]] 2.16Å' scene=''> | + | <StructureSection load='4glk' size='340' side='right'caption='[[4glk]], [[Resolution|resolution]] 2.16Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4glk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_lactis"_lister_1873 "bacterium lactis" lister 1873]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GLK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GLK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4glk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GLK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GLK FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">abiQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 "Bacterium lactis" Lister 1873])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4glk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4glk OCA], [https://pdbe.org/4glk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4glk RCSB], [https://www.ebi.ac.uk/pdbsum/4glk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4glk ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4glk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4glk OCA], [http://pdbe.org/4glk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4glk RCSB], [http://www.ebi.ac.uk/pdbsum/4glk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4glk ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/ABIQ_LACLL ABIQ_LACLL] Toxic component of a type III toxin-antitoxin (TA) system. An endoribonuclease that is probably sequence-specific. It is neutralized by its cognate antitoxin RNA AntiQ, which has 2.8 35 nucleotide-long repeats. Cannot be cloned in L.lactis subsp. cremoris strain NZ9000 in the absence of the antitoxin gene; expression in strain NZ9000 even in the presence of antiQ inhibits growth in a bacteriostatic fashion (PubMed:23279123). Confers resistance to 936 and c2 phages but not P335 phages in L.lactis, causes an abortive infection (Abi phenotype). Viral DNA is replicated but not cleaved from its concatemeric form, while the viral major structural protein is produced normally in the presence of this protein (PubMed:9835558). Operon expression in E.coli confers resistance to 3 phages of the Myoviridae family (T4, RB69 and phage 2) and 1 of the Siphoviridae family (T5), but not other tested phages (T1, T3, lambda vir, HK97, Mu and pilH alpha). The presence of this operon in L.lactis subsp. lactis strain IL1403 during phage P008 infection alters the viral transcription profiles (PubMed:23813728).<ref>PMID:23279123</ref> <ref>PMID:23813728</ref> <ref>PMID:9835558</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterium lactis lister 1873]] | + | [[Category: Lactococcus lactis]] |
| - | [[Category: Cambillau, C]] | + | [[Category: Large Structures]] |
| - | [[Category: Moineau, S]] | + | [[Category: Cambillau C]] |
| - | [[Category: Samson, J]] | + | [[Category: Moineau S]] |
| - | [[Category: Spinelli, S]] | + | [[Category: Samson J]] |
| - | [[Category: Alpha beta]]
| + | [[Category: Spinelli S]] |
| - | [[Category: Endonuclease]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Rna antiq]]
| + | |
| Structural highlights
Function
ABIQ_LACLL Toxic component of a type III toxin-antitoxin (TA) system. An endoribonuclease that is probably sequence-specific. It is neutralized by its cognate antitoxin RNA AntiQ, which has 2.8 35 nucleotide-long repeats. Cannot be cloned in L.lactis subsp. cremoris strain NZ9000 in the absence of the antitoxin gene; expression in strain NZ9000 even in the presence of antiQ inhibits growth in a bacteriostatic fashion (PubMed:23279123). Confers resistance to 936 and c2 phages but not P335 phages in L.lactis, causes an abortive infection (Abi phenotype). Viral DNA is replicated but not cleaved from its concatemeric form, while the viral major structural protein is produced normally in the presence of this protein (PubMed:9835558). Operon expression in E.coli confers resistance to 3 phages of the Myoviridae family (T4, RB69 and phage 2) and 1 of the Siphoviridae family (T5), but not other tested phages (T1, T3, lambda vir, HK97, Mu and pilH alpha). The presence of this operon in L.lactis subsp. lactis strain IL1403 during phage P008 infection alters the viral transcription profiles (PubMed:23813728).[1] [2] [3]
Publication Abstract from PubMed
AbiQ is a phage resistance mechanism found on a native plasmid of Lactococcus lactis that abort virulent phage infections. In this study, we experimentally demonstrate that AbiQ belongs to the recently described type III toxin-antitoxin systems. When overexpressed, the AbiQ protein (ABIQ) is toxic and causes bacterial death in a bacteriostatic manner. Northern and Western blot experiments revealed that the abiQ gene is transcribed and translated constitutively, and its expression is not activated by a phage product. ABIQ is an endoribonuclease that specifically cleaves its cognate antitoxin RNA molecule in vivo. The crystal structure of ABIQ was solved and site-directed mutagenesis identified key amino acids for its anti-phage and/or its RNase function. The AbiQ system is the first lactococcal abortive infection system characterized to date at a structural level.
Structure and activity of AbiQ, a lactococcal endoribonuclease belonging to the type III toxin-antitoxin system.,Samson JE, Spinelli S, Cambillau C, Moineau S Mol Microbiol. 2012 Dec 20. doi: 10.1111/mmi.12129. PMID:23279123[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Samson JE, Spinelli S, Cambillau C, Moineau S. Structure and activity of AbiQ, a lactococcal endoribonuclease belonging to the type III toxin-antitoxin system. Mol Microbiol. 2012 Dec 20. doi: 10.1111/mmi.12129. PMID:23279123 doi:http://dx.doi.org/10.1111/mmi.12129
- ↑ Samson JE, Belanger M, Moineau S. Effect of the abortive infection mechanism and type III toxin/antitoxin system AbiQ on the lytic cycle of Lactococcus lactis phages. J Bacteriol. 2013 Sep;195(17):3947-56. doi: 10.1128/JB.00296-13. PMID:23813728 doi:http://dx.doi.org/10.1128/JB.00296-13
- ↑ Emond E, Dion E, Walker SA, Vedamuthu ER, Kondo JK, Moineau S. AbiQ, an abortive infection mechanism from Lactococcus lactis. Appl Environ Microbiol. 1998 Dec;64(12):4748-56. doi:, 10.1128/AEM.64.12.4748-4756.1998. PMID:9835558 doi:http://dx.doi.org/10.1128/AEM.64.12.4748-4756.1998
- ↑ Samson JE, Spinelli S, Cambillau C, Moineau S. Structure and activity of AbiQ, a lactococcal endoribonuclease belonging to the type III toxin-antitoxin system. Mol Microbiol. 2012 Dec 20. doi: 10.1111/mmi.12129. PMID:23279123 doi:http://dx.doi.org/10.1111/mmi.12129
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