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| | ==Structural basis of Rpl5 recognition by Syo1== | | ==Structural basis of Rpl5 recognition by Syo1== |
| - | <StructureSection load='4gmn' size='340' side='right' caption='[[4gmn]], [[Resolution|resolution]] 2.95Å' scene=''> | + | <StructureSection load='4gmn' size='340' side='right'caption='[[4gmn]], [[Resolution|resolution]] 2.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4gmn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GMN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GMN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4gmn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GMN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GMN FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0063640, Rpl5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD]), CTHT_0033460, Syo1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gmn OCA], [https://pdbe.org/4gmn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gmn RCSB], [https://www.ebi.ac.uk/pdbsum/4gmn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gmn ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gmn OCA], [http://pdbe.org/4gmn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gmn RCSB], [http://www.ebi.ac.uk/pdbsum/4gmn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gmn ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/G0SEG2_CHATD G0SEG2_CHATD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chatd]] | + | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] |
| - | [[Category: Bange, G]] | + | [[Category: Large Structures]] |
| - | [[Category: Sinning, I]] | + | [[Category: Bange G]] |
| - | [[Category: Arm]] | + | [[Category: Sinning I]] |
| - | [[Category: Chaperone]]
| + | |
| - | [[Category: Heat]]
| + | |
| - | [[Category: Kap104]]
| + | |
| - | [[Category: Linear motif]]
| + | |
| - | [[Category: Nuclear transport]]
| + | |
| - | [[Category: Ribosome assembly]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rpl11]]
| + | |
| - | [[Category: Solenoid]]
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| Structural highlights
Function
G0SEG2_CHATD
Publication Abstract from PubMed
Ribosomal proteins are synthesized in the cytoplasm, before nuclear import and assembly with ribosomal RNA (rRNA). Little is known about coordination of nucleocytoplasmic transport with ribosome assembly. Here, we identify a transport adaptor, symportin 1 (Syo1), that facilitates synchronized coimport of the two 5S-rRNA binding proteins Rpl5 and Rpl11. In vitro studies revealed that Syo1 concomitantly binds Rpl5-Rpl11 and furthermore recruits the import receptor Kap104. The Syo1-Rpl5-Rpl11 import complex is released from Kap104 by RanGTP and can be directly transferred onto the 5S rRNA. Syo1 can shuttle back to the cytoplasm by interaction with phenylalanine-glycine nucleoporins. X-ray crystallography uncovered how the alpha-solenoid symportin accommodates the Rpl5 amino terminus, normally bound to 5S rRNA, in an extended groove. Symportin-mediated coimport of Rpl5-Rpl11 could ensure coordinated and stoichiometric incorporation of these proteins into pre-60S ribosomes.
Synchronizing nuclear import of ribosomal proteins with ribosome assembly.,Kressler D, Bange G, Ogawa Y, Stjepanovic G, Bradatsch B, Pratte D, Amlacher S, Strauss D, Yoneda Y, Katahira J, Sinning I, Hurt E Science. 2012 Nov 2;338(6107):666-71. doi: 10.1126/science.1226960. PMID:23118189[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kressler D, Bange G, Ogawa Y, Stjepanovic G, Bradatsch B, Pratte D, Amlacher S, Strauss D, Yoneda Y, Katahira J, Sinning I, Hurt E. Synchronizing nuclear import of ribosomal proteins with ribosome assembly. Science. 2012 Nov 2;338(6107):666-71. doi: 10.1126/science.1226960. PMID:23118189 doi:http://dx.doi.org/10.1126/science.1226960
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