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1itz

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[[Image:1itz.gif|left|200px]]
[[Image:1itz.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1itz |SIZE=350|CAPTION= <scene name='initialview01'>1itz</scene>, resolution 2.3&Aring;
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The line below this paragraph, containing "STRUCTURE_1itz", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1itz| PDB=1itz | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1itz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1itz OCA], [http://www.ebi.ac.uk/pdbsum/1itz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1itz RCSB]</span>
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}}
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'''Maize Transketolase in complex with TPP'''
'''Maize Transketolase in complex with TPP'''
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[[Category: Gerhardt, S.]]
[[Category: Gerhardt, S.]]
[[Category: Huber, R.]]
[[Category: Huber, R.]]
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[[Category: calvin cycle]]
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[[Category: Calvin cycle]]
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[[Category: cofactor]]
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[[Category: Cofactor]]
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[[Category: plant]]
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[[Category: Plant]]
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[[Category: thiamine pyrophosphate]]
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[[Category: Thiamine pyrophosphate]]
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[[Category: transketolase]]
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[[Category: Transketolase]]
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[[Category: zea may]]
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[[Category: Zea may]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:24:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:24:35 2008''
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Revision as of 17:24, 2 May 2008

Image:1itz.gif

Template:STRUCTURE 1itz

Maize Transketolase in complex with TPP


Overview

The gene specifying plastid transketolase (TK) of maize (Zea mays) was cloned from a cDNA library by southern blotting using a heterologous probe from sorghum (Sorghum bicolor). A recombinant fusion protein comprising thioredoxin of Escherichia coli and mature TK of maize was expressed at a high level in E. coli and cleaved with thrombin, affording plastid TK. The protein in complex with thiamine pyrophoshate was crystallized, and its structure was solved by molecular replacement. The enzyme is a C2 symmetric homodimer closely similar to the enzyme from yeast (Saccharomyces cerevisiae). Each subunit is folded into three domains. The two topologically equivalent active sites are located in the subunit interface region and resemble those of the yeast enzyme.

About this Structure

1ITZ is a Single protein structure of sequence from Zea mays. Full crystallographic information is available from OCA.

Reference

Structure and properties of an engineered transketolase from maize., Gerhardt S, Echt S, Busch M, Freigang J, Auerbach G, Bader G, Martin WF, Bacher A, Huber R, Fischer M, Plant Physiol. 2003 Aug;132(4):1941-9. PMID:12913150 Page seeded by OCA on Fri May 2 20:24:39 2008

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