7o13

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m (Protected "7o13" [edit=sysop:move=sysop])
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'''Unreleased structure'''
 
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The entry 7o13 is ON HOLD until sometime in the future
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==ABC transporter NosDFY, nucleotide-free in lipid nanodisc==
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<StructureSection load='7o13' size='340' side='right'caption='[[7o13]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[7o13]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_stutzeri_ATCC_14405_=_CCUG_16156 Pseudomonas stutzeri ATCC 14405 = CCUG 16156]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O13 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o13 OCA], [https://pdbe.org/7o13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o13 RCSB], [https://www.ebi.ac.uk/pdbsum/7o13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o13 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NOSD_PSEST NOSD_PSEST] Required for the assembly of the copper chromophores of nitrous oxide reductase (PubMed:2170125). Could be part of the ABC transporter complex NosDFY (Probable).<ref>PMID:2170125</ref> <ref>PMID:12618453</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Emissions of the critical ozone-depleting and greenhouse gas nitrous oxide (N2O) from soils and industrial processes have increased considerably over the last decades(1-3). As the final step of bacterial denitrification, N2O is reduced to chemically inert N2 (refs. (1,4)) in a reaction that is catalysed by the copper-dependent nitrous oxide reductase (N2OR) (ref. (5)). The assembly of its unique [4Cu:2S] active site cluster CuZ requires both the ATP-binding-cassette (ABC) complex NosDFY and the membrane-anchored copper chaperone NosL (refs. (4,6)). Here we report cryo-electron microscopy structures of Pseudomonas stutzeri NosDFY and its complexes with NosL and N2OR, respectively. We find that the periplasmic NosD protein contains a binding site for a Cu(+) ion and interacts specifically with NosL in its nucleotide-free state, whereas its binding to N2OR requires a conformational change that is triggered by ATP binding. Mutually exclusive structures of NosDFY in complex with NosL and with N2OR reveal a sequential metal-trafficking and assembly pathway for a highly complex copper site. Within this pathway, NosDFY acts as a mechanical energy transducer rather than as a transporter. It links ATP hydrolysis in the cytoplasm to a conformational transition of the NosD subunit in the periplasm, which is required for NosDFY to switch its interaction partner so that copper ions are handed over from the chaperone NosL to the enzyme N2OR.
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Authors:
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Molecular interplay of an assembly machinery for nitrous oxide reductase.,Muller C, Zhang L, Zipfel S, Topitsch A, Lutz M, Eckert J, Prasser B, Chami M, Lu W, Du J, Einsle O Nature. 2022 Aug;608(7923):626-631. doi: 10.1038/s41586-022-05015-2. Epub 2022, Jul 27. PMID:35896743<ref>PMID:35896743</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 7o13" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pseudomonas stutzeri ATCC 14405 = CCUG 16156]]
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[[Category: Du J]]
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[[Category: Einsle O]]
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[[Category: Lu W]]
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[[Category: Mueller C]]
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[[Category: Zhang L]]

Revision as of 07:11, 3 November 2022

ABC transporter NosDFY, nucleotide-free in lipid nanodisc

PDB ID 7o13

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