1hap
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(New page: 200px<br /> <applet load="1hap" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hap, resolution 2.800Å" /> '''COMPLEX OF HUMAN A...)
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Revision as of 15:08, 12 November 2007
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COMPLEX OF HUMAN ALPHA-THROMBIN WITH A 15MER OLIGONUCLEOTIDE GGTTGGTGTGGTTGG (BASED ON X-RAY MODEL OF DNA)
Contents |
Overview
The structure of a complex between thrombin and a GGTTGGTGTGGTTGG DNA, 15-mer has been analyzed crystallographically. The solution NMR structure, of the 15-mer has two stacked G-quartets similar to that found in the, previous X-ray structure determination of the 15-mer-thrombin complex, [Padmanabhan, Padmanabhan, Ferrara, Sadler & Tulinsky (1993). J. Biol., Chem. 268, 17651-17654]; the strand polarity, however, is reversed from, that of the crystallographic structure. The structure of the complex here, has been redetermined with better diffraction data confirming the previous, crystallographic structure but also indicating that the NMR solution, structure fits equally well. Both 15-mer complex structures refined to an, R value of about 0.16 presenting a disconcerting ambiguity. Since the two, 15-mer structures associate with thrombin in different ways (through the, TGT loop in the X-ray and TT loop in the NMR model), other independent, lines of physical or chemical evidence are required to resolve the, ambiguity.
Disease
Known diseases associated with this structure: Dysprothrombinemia OMIM:[176930], Hyperprothrombinemia OMIM:[176930], Hypoprothrombinemia OMIM:[176930]
About this Structure
1HAP is a Single protein structure of sequence from Homo sapiens with CH2 as ligand. Full crystallographic information is available from OCA.
Reference
An ambiguous structure of a DNA 15-mer thrombin complex., Padmanabhan K, Tulinsky A, Acta Crystallogr D Biol Crystallogr. 1996 Mar 1;52(Pt 2):272-82. PMID:15299700
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