8ad8
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Flavin-dependent tryptophan 6-halogenase Thal in complex with a D-Trp-Ser dipeptide== | |
| + | <StructureSection load='8ad8' size='340' side='right'caption='[[8ad8]], [[Resolution|resolution]] 2.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8ad8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_albogriseolus Streptomyces albogriseolus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8AD8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8AD8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LT0:D-tryptophyl-L-serine'>LT0</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ad8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ad8 OCA], [https://pdbe.org/8ad8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ad8 RCSB], [https://www.ebi.ac.uk/pdbsum/8ad8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ad8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A1E280_STRAO A1E280_STRAO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The late-stage site-selective derivatisation of peptides has many potential applications in structure-activity relationship studies and postsynthetic modification or conjugation of bioactive compounds. The development of orthogonal methods for C-H functionalisation is crucial for such peptide derivatisation. Among them biocatalytic methods are increasingly attracting attention. Tryptophan halogenases emerged as valuable catalysts to functionalise tryptophan (Trp), while direct enzyme-catalysed halogenation of synthetic peptides is yet unprecedented. Here, it is reported that the Trp 6-halogenase Thal accepts a wide range of amides and peptides containing a Trp moiety. Increasing the sequence length and reaction optimisation made bromination of pentapeptides feasible with good turnovers and a broad sequence scope, while regioselectivity turned out to be sequence dependent. Comparison of X-ray single crystal structures of Thal in complex with d-Trp and a dipeptide revealed a significantly altered binding mode for the peptide. The viability of this bioorthogonal approach was exemplified by halogenation of a cyclic RGD peptide. | ||
| - | + | Enzymatic Late-Stage Halogenation of Peptides.,Schnepel C, Moritzer AC, Gafe S, Montua N, Minges H, Niess A, Niemann HH, Sewald N Chembiochem. 2022 Oct 19. doi: 10.1002/cbic.202200569. PMID:36259362<ref>PMID:36259362</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8ad8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptomyces albogriseolus]] | ||
| + | [[Category: Gaefe S]] | ||
| + | [[Category: Montua N]] | ||
| + | [[Category: Moritzer AC]] | ||
| + | [[Category: Niemann HH]] | ||
| + | [[Category: Sewald N]] | ||
Revision as of 07:36, 3 November 2022
Flavin-dependent tryptophan 6-halogenase Thal in complex with a D-Trp-Ser dipeptide
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