4h67

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(Difference between revisions)

Revision as of 08:01, 3 November 2022

Crystal structure of HMP synthase Thi5 from S. cerevisiae

Structural highlights

4h67 is a 8 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

THI5_YEAST Proposed biosynthetic enzyme involved in pyrimidine biosynthesis pathway above the hydroxymethyl-pyrimidine precursor leading to the thiamine moiety.^[1]

Publication Abstract from PubMed

Vitamin B1 is essential for all organisms being well recognized as a necessary cofactor for key metabolic pathways such as glycolysis and was more recently implicated in DNA damage responses. Little is known about the enzyme responsible for the formation of the pyrimidine moiety (HMP-P synthase). We report a structure-function study of the HMP-P synthase from yeast, THI5p. Our crystallographic structure shows that the THI5p is a mix between periplasmic binding proteins and pyridoxal 5'-phosphate (PLP) dependent enzymes. Mutational and yeast complementation studies identify the key residues for HMP-P biosynthesis and offers unprecedented insights into the use of PLP as a substrate rather than as a cofactor, the requirement of iron and more generally into the HMP-P synthase reaction mechanism.

The last piece in the vitamin B1 biosynthesis puzzle: Structural and functional insight into yeast HMP-P synthase.,Coquille S, Roux C, Fitzpatrick TB, Thore S J Biol Chem. 2012 Oct 9. PMID:23048037^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wightman R, Meacock PA. The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine. Microbiology. 2003 Jun;149(Pt 6):1447-60. PMID:12777485
↑ Coquille S, Roux C, Fitzpatrick TB, Thore S. The last piece in the vitamin B1 biosynthesis puzzle: Structural and functional insight into yeast HMP-P synthase. J Biol Chem. 2012 Oct 9. PMID:23048037 doi:http://dx.doi.org/10.1074/jbc.M112.397240

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4h67"

@@ Line 1: / Line 1: @@
 ==Crystal structure of HMP synthase Thi5 from S. cerevisiae==
-<StructureSection load='4h67' size='340' side='right' caption='[[4h67]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='4h67' size='340' side='right'caption='[[4h67]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4h67]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H67 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4h67]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H67 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4h65|4h65]], [[4h6d|4h6d]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h67 OCA], [https://pdbe.org/4h67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h67 RCSB], [https://www.ebi.ac.uk/pdbsum/4h67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h67 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">THI5, YFL058W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h67 OCA], [http://pdbe.org/4h67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h67 RCSB], [http://www.ebi.ac.uk/pdbsum/4h67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h67 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/THI5_YEAST THI5_YEAST]] Proposed biosynthetic enzyme involved in pyrimidine biosynthesis pathway above the hydroxymethyl-pyrimidine precursor leading to the thiamine moiety.<ref>PMID:12777485</ref>
+[https://www.uniprot.org/uniprot/THI5_YEAST THI5_YEAST] Proposed biosynthetic enzyme involved in pyrimidine biosynthesis pathway above the hydroxymethyl-pyrimidine precursor leading to the thiamine moiety.<ref>PMID:12777485</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 18824]]
+[[Category: Large Structures]]
-[[Category: Coquille, S C]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Fitzpatrick, T]]
+[[Category: Coquille SC]]
-[[Category: Roux, C]]
+[[Category: Fitzpatrick T]]
-[[Category: Thore, S]]
+[[Category: Roux C]]
-[[Category: Hmp-p synthase]]
+[[Category: Thore S]]
-[[Category: Plp binding]]
-[[Category: Thi5-plp complex]]
-[[Category: Transferase]]

4h67

From Proteopedia

Revision as of 08:01, 3 November 2022

Crystal structure of HMP synthase Thi5 from S. cerevisiae

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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