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| | ==Crystal structure of CasB from Thermus thermophilus== | | ==Crystal structure of CasB from Thermus thermophilus== |
| - | <StructureSection load='4h7a' size='340' side='right' caption='[[4h7a]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='4h7a' size='340' side='right'caption='[[4h7a]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4h7a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H7A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H7A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4h7a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H7A FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cse2, TTHB189 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h7a OCA], [https://pdbe.org/4h7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h7a RCSB], [https://www.ebi.ac.uk/pdbsum/4h7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h7a ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h7a OCA], [http://pdbe.org/4h7a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h7a RCSB], [http://www.ebi.ac.uk/pdbsum/4h7a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h7a ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CSE2_THET8 CSE2_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). | + | [https://www.uniprot.org/uniprot/CSE2_THET8 CSE2_THET8] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4h7a" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4h7a" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[CRISPR type I-E (Cascade)|CRISPR type I-E (Cascade)]] |
| | + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Thet8]] | + | [[Category: Large Structures]] |
| - | [[Category: Ke, A]] | + | [[Category: Thermus thermophilus HB8]] |
| - | [[Category: Nam, K H]] | + | [[Category: Ke A]] |
| - | [[Category: Casb]] | + | [[Category: Nam KH]] |
| - | [[Category: Cascade]]
| + | |
| - | [[Category: Crispr]]
| + | |
| - | [[Category: Crispr-assoicated protein]]
| + | |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Nucleic acid binding protein]]
| + | |
| Structural highlights
Function
CSE2_THET8 CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity).
Publication Abstract from PubMed
The CRISPR system is an adaptive RNA-based microbial immune system against invasive genetic elements. CasB is an essential protein component in Type I-E Cascade. Here, we characterize CasB proteins from three different organisms as non-specific nucleic acid binding proteins. The Thermobifida fusca CasB crystal structure reveals conserved positive surface charges, which we show are important for its nucleic acid binding function. EM docking reveals that CasB dimerization aligns individual nucleic acid binding surfaces into a curved, elongated binding surface inside Type I-E Cascade, consistent with the putative functions of CasB in ds-DNA recruitment and crRNA-DNA duplex formation steps. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: TthCasB and TthCasBbind by x-ray crystallography (View interaction) TfuCasB1 and TfuCasB1bind by molecular sieving (View Interaction: 1, 2).
Nucleic acid binding surface and dimer interface revealed by CRISPR-associated CasB protein structures.,Nam KH, Huang Q, Ke A FEBS Lett. 2012 Nov 16;586(22):3956-61. doi: 10.1016/j.febslet.2012.09.041. Epub , 2012 Oct 16. PMID:23079036[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Nam KH, Huang Q, Ke A. Nucleic acid binding surface and dimer interface revealed by CRISPR-associated CasB protein structures. FEBS Lett. 2012 Nov 16;586(22):3956-61. doi: 10.1016/j.febslet.2012.09.041. Epub , 2012 Oct 16. PMID:23079036 doi:http://dx.doi.org/10.1016/j.febslet.2012.09.041
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