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| | ==Structural insights into the reduction mechanism of Saccharomyces cerevisia Riboflavin Biosynthesis Reductase Rib7== | | ==Structural insights into the reduction mechanism of Saccharomyces cerevisia Riboflavin Biosynthesis Reductase Rib7== |
| - | <StructureSection load='4ha7' size='340' side='right' caption='[[4ha7]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='4ha7' size='340' side='right'caption='[[4ha7]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ha7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HA7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HA7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ha7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HA7 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ha9|4ha9]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ha7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ha7 OCA], [https://pdbe.org/4ha7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ha7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ha7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ha7 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RIB7, YBR153W, YBR1203 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
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| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone_5'-phosphate_reductase 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.302 1.1.1.302] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ha7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ha7 OCA], [http://pdbe.org/4ha7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ha7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ha7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ha7 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RIB7_YEAST RIB7_YEAST]] Catalyzes an early step in riboflavin biosynthesis, the NADPH-dependent reduction of the ribose side chain of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate, yielding 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate (Probable). | + | [https://www.uniprot.org/uniprot/RIB7_YEAST RIB7_YEAST] Catalyzes an early step in riboflavin biosynthesis, the NADPH-dependent reduction of the ribose side chain of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate, yielding 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate (Probable). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | + | [[Category: Large Structures]] |
| - | [[Category: Liu, Y]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Lv, Z]] | + | [[Category: Liu Y]] |
| - | [[Category: Sun, J]] | + | [[Category: Lv Z]] |
| - | [[Category: Nadph binding]] | + | [[Category: Sun J]] |
| - | [[Category: Oxidoreductase]]
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| - | [[Category: Reductase]]
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| Structural highlights
Function
RIB7_YEAST Catalyzes an early step in riboflavin biosynthesis, the NADPH-dependent reduction of the ribose side chain of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate, yielding 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate (Probable).
Publication Abstract from PubMed
Saccharomyces cerevisiae RIB7 (ScRIB7) is a potent target for anti-fungal agents because of its involvement in the riboflavin biosynthesis pathway as a NADPH-dependent reductase. However, the catalytic mechanism of riboflavin biosynthesis reductase (RBSRs) is controversial, and enzyme structure information is still lacking in eukaryotes. Here we report the crystal structure of Saccharomyces cerevisiae RIB7 at 2.10 A resolution and its complex with NADPH at 2.35 A resolution. ScRIB7 exists as a stable homodimer, and each subunit consists of nine central beta-sheets flanked by five helices, resembling the structure of RIB7 homologues. A conserved G(76)-X-G(78)-Xn-G(181)-G(182) motif is present at the NADPH pyrophosphate group binding site. Activity assays confirmed the necessity of Thr79, Asp83, Glu180 and Gly182 for the activity of ScRIB7. Substrate preference of ScRIB7 was altered by mutating one residue (Thr35) to a Lysine, implying that ScRIB7 Thr35 and its corresponding residue, a lysine in bacteria, are important in substrate-specific recognition.
Structural and functional insights into Saccharomyces cerevisiae riboflavin biosynthesis reductase RIB7.,Lv Z, Sun J, Liu Y PLoS One. 2013 Apr 19;8(4):e61249. doi: 10.1371/journal.pone.0061249. Print 2013. PMID:23620735[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lv Z, Sun J, Liu Y. Structural and functional insights into Saccharomyces cerevisiae riboflavin biosynthesis reductase RIB7. PLoS One. 2013 Apr 19;8(4):e61249. doi: 10.1371/journal.pone.0061249. Print 2013. PMID:23620735 doi:10.1371/journal.pone.0061249
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