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| | ==Structure of the AppA-PpsR2 core complex from Rb. sphaeroides== | | ==Structure of the AppA-PpsR2 core complex from Rb. sphaeroides== |
| - | <StructureSection load='4hh3' size='340' side='right' caption='[[4hh3]], [[Resolution|resolution]] 1.75Å' scene=''> | + | <StructureSection load='4hh3' size='340' side='right'caption='[[4hh3]], [[Resolution|resolution]] 1.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4hh3]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhodococcus_capsulatus"_molisch_1907 "rhodococcus capsulatus" molisch 1907] and [http://en.wikipedia.org/wiki/Rhos4 Rhos4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HH3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HH3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4hh3]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides] and [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides_2.4.1 Cereibacter sphaeroides 2.4.1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HH3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HH3 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hh2|4hh2]], [[4hh0|4hh0]], [[4hh1|4hh1]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hh3 OCA], [https://pdbe.org/4hh3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hh3 RCSB], [https://www.ebi.ac.uk/pdbsum/4hh3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hh3 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ppsR, RHOS4_18870, RSP_0282 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272943 RHOS4]), appA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 "Rhodococcus capsulatus" Molisch 1907])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hh3 OCA], [http://pdbe.org/4hh3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hh3 RCSB], [http://www.ebi.ac.uk/pdbsum/4hh3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hh3 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q3J179_CERS4 Q3J179_CERS4] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4hh3" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4hh3" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[AppA protein BLUF domain|AppA protein BLUF domain]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Rhodococcus capsulatus molisch 1907]] | + | [[Category: Cereibacter sphaeroides]] |
| - | [[Category: Rhos4]] | + | [[Category: Cereibacter sphaeroides 2 4.1]] |
| - | [[Category: Heintz, U]] | + | [[Category: Large Structures]] |
| - | [[Category: Lindner, R]] | + | [[Category: Heintz U]] |
| - | [[Category: Reinstein, J]] | + | [[Category: Lindner R]] |
| - | [[Category: Schlichting, I]] | + | [[Category: Reinstein J]] |
| - | [[Category: Shoeman, R]] | + | [[Category: Schlichting I]] |
| - | [[Category: Winkler, A]] | + | [[Category: Shoeman R]] |
| - | [[Category: Appa-ppsr complex]]
| + | [[Category: Winkler A]] |
| - | [[Category: Flavoprotein-transcription complex]]
| + | |
| - | [[Category: Helix bundle]]
| + | |
| - | [[Category: Pas domain]]
| + | |
| - | [[Category: Schic domain]]
| + | |
| Structural highlights
Function
Q3J179_CERS4
Publication Abstract from PubMed
The anoxygenic phototrophic bacterium Rhodobacter sphaeroides uses different energy sources, depending on environmental conditions including aerobic respiration or, in the absence of oxygen, photosynthesis. Photosynthetic genes are repressed at high oxygen tension, but at intermediate levels their partial expression prepares the bacterium for using light energy. Illumination, however, enhances repression under semiaerobic conditions. Here, we describe molecular details of two proteins mediating oxygen and light control of photosynthesis-gene expression: the light-sensing antirepressor AppA and the transcriptional repressor PpsR. Our crystal structures of both proteins and their complex and hydrogen/deuterium-exchange data show that light activation of AppA-PpsR2 affects the PpsR effector region within the complex. DNA binding studies demonstrate the formation of a light-sensitive ternary AppA-PpsR-DNA complex. We discuss implications of these results for regulation by light and oxygen, highlighting new insights into blue light-mediated signal transduction.
A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression.,Winkler A, Heintz U, Lindner R, Reinstein J, Shoeman RL, Schlichting I Nat Struct Mol Biol. 2013 Jul;20(7):859-67. doi: 10.1038/nsmb.2597. Epub 2013 Jun, 2. PMID:23728293[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Winkler A, Heintz U, Lindner R, Reinstein J, Shoeman RL, Schlichting I. A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression. Nat Struct Mol Biol. 2013 Jul;20(7):859-67. doi: 10.1038/nsmb.2597. Epub 2013 Jun, 2. PMID:23728293 doi:10.1038/nsmb.2597
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