1ixy
From Proteopedia
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[[Image:1ixy.gif|left|200px]] | [[Image:1ixy.gif|left|200px]] | ||
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'''Ternary complex of T4 phage BGT with UDP and a 13 mer DNA duplex''' | '''Ternary complex of T4 phage BGT with UDP and a 13 mer DNA duplex''' | ||
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[[Category: Lariviere, L.]] | [[Category: Lariviere, L.]] | ||
[[Category: Morera, S.]] | [[Category: Morera, S.]] | ||
| - | [[Category: | + | [[Category: Abasic site]] |
| - | [[Category: | + | [[Category: Base-flipping]] |
| - | [[Category: | + | [[Category: Protein-dna complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:33:49 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:33, 2 May 2008
Ternary complex of T4 phage BGT with UDP and a 13 mer DNA duplex
Overview
T4 phage beta-glucosyltransferase (BGT) modifies T4 DNA. We crystallized BGT with UDP-glucose and a 13mer DNA fragment containing an abasic site. We obtained two crystal structures of a ternary complex BGT-UDP-DNA at 1.8A and 2.5A resolution, one with a Tris molecule and the other with a metal ion at the active site. Both structures reveal a large distortion in the bound DNA. BGT flips the deoxyribose moiety at the abasic site to an extra-helical position and induces a 40 degrees bend in the DNA with a marked widening of the major groove. The Tris molecule mimics the glucose moiety in its transition state. The base-flipping mechanism, which has so far been observed only for glycosylases, methyltransferases and endonucleases, is now reported for a glucosyltransferase. BGT is unique in binding and inserting a loop into the DNA duplex through the major groove only. Furthermore, BGT compresses the backbone DNA one base further than the target base on the 3'-side.
About this Structure
1IXY is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
A base-flipping mechanism for the T4 phage beta-glucosyltransferase and identification of a transition-state analog., Lariviere L, Morera S, J Mol Biol. 2002 Nov 29;324(3):483-90. PMID:12445783 Page seeded by OCA on Fri May 2 20:33:49 2008
