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| | ==Crystallographic structure of the membrane-proximal ectodomain of the human receptor-type protein-tyrosine phosphatase phogrin== | | ==Crystallographic structure of the membrane-proximal ectodomain of the human receptor-type protein-tyrosine phosphatase phogrin== |
| - | <StructureSection load='4hti' size='340' side='right' caption='[[4hti]], [[Resolution|resolution]] 1.95Å' scene=''> | + | <StructureSection load='4hti' size='340' side='right'caption='[[4hti]], [[Resolution|resolution]] 1.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4hti]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HTI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HTI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4hti]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HTI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HTI FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PTPRN2, KIAA0387 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hti OCA], [https://pdbe.org/4hti PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hti RCSB], [https://www.ebi.ac.uk/pdbsum/4hti PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hti ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hti OCA], [http://pdbe.org/4hti PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hti RCSB], [http://www.ebi.ac.uk/pdbsum/4hti PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hti ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PTPR2_HUMAN PTPR2_HUMAN]] Implicated in development of nervous system and pancreatic endocrine cells.<ref>PMID:8878534</ref> <ref>PMID:8798755</ref> | + | [https://www.uniprot.org/uniprot/PTPR2_HUMAN PTPR2_HUMAN] Implicated in development of nervous system and pancreatic endocrine cells.<ref>PMID:8878534</ref> <ref>PMID:8798755</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[3D structures of antibody|3D structures of antibody]] | + | *[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Protein-tyrosine-phosphatase]] | + | [[Category: Large Structures]] |
| - | [[Category: Ermacora, M R]] | + | [[Category: Ermacora MR]] |
| - | [[Category: Jakoncic, J]] | + | [[Category: Jakoncic J]] |
| - | [[Category: Noguera, M E]] | + | [[Category: Noguera ME]] |
| - | [[Category: Poskus, E]] | + | [[Category: Poskus E]] |
| - | [[Category: Autoimmunity]]
| + | |
| - | [[Category: Diabetes]]
| + | |
| - | [[Category: Ferredoxin-like fold]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Ia-2beta]]
| + | |
| - | [[Category: Phogrin]]
| + | |
| - | [[Category: Protein-tyrosine phosphatase]]
| + | |
| - | [[Category: Receptor]]
| + | |
| - | [[Category: Transmembrane protein]]
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| Structural highlights
Function
PTPR2_HUMAN Implicated in development of nervous system and pancreatic endocrine cells.[1] [2]
Publication Abstract from PubMed
Phogrin/IA-2beta and ICA512/IA-2 are two paralogs receptor-type protein-tyrosine phosphatases (RPTP) that localize in secretory granules of various neuroendocrine cells. In pancreatic islet beta-cells, they participate in the regulation of insulin secretion, ensuring proper granulogenesis, and beta-cell proliferation. The role of their cytoplasmic tail has been partially unveiled, while that of their luminal region remains unclear. To advance the understanding of its structure-function relationship, the X-ray structure of the mature ectodomain of phogrin (ME phogrin) at pH 7.4 and 4.6 has been solved at 1.95- and 2.01-A resolution, respectively. Similarly to the ME of ICA512, ME phogrin adopts a ferredoxin-like fold: a sheet of four antiparallel beta-strands packed against two alpha-helices. Sequence conservation among vertebrates, plants and insects suggests that the structural similarity extends to all the receptor family. Crystallized ME phogrin is monomeric, in agreement with solution studies but in striking contrast with the behavior of homodimeric ME ICA512. The structural details that may cause the quaternary structure differences are analyzed. The results provide a basis for building models of the overall orientation and oligomerization state of the receptor in biological membranes.
X-ray structure of the mature ectodomain of phogrin.,Noguera ME, Primo ME, Jakoncic J, Poskus E, Solimena M, Ermacora MR J Struct Funct Genomics. 2015 Mar;16(1):1-9. doi: 10.1007/s10969-014-9191-0. Epub, 2014 Nov 26. PMID:25421040[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kawasaki E, Hutton JC, Eisenbarth GS. Molecular cloning and characterization of the human transmembrane protein tyrosine phosphatase homologue, phogrin, an autoantigen of type 1 diabetes. Biochem Biophys Res Commun. 1996 Oct 14;227(2):440-7. PMID:8878534 doi:S0006-291X(96)91526-0
- ↑ Cui L, Yu WP, DeAizpurua HJ, Schmidli RS, Pallen CJ. Cloning and characterization of islet cell antigen-related protein-tyrosine phosphatase (PTP), a novel receptor-like PTP and autoantigen in insulin-dependent diabetes. J Biol Chem. 1996 Oct 4;271(40):24817-23. PMID:8798755
- ↑ Noguera ME, Primo ME, Jakoncic J, Poskus E, Solimena M, Ermacora MR. X-ray structure of the mature ectodomain of phogrin. J Struct Funct Genomics. 2015 Mar;16(1):1-9. doi: 10.1007/s10969-014-9191-0. Epub, 2014 Nov 26. PMID:25421040 doi:http://dx.doi.org/10.1007/s10969-014-9191-0
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