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| | ==Crystal structure of LdtMt2, a L,D-transpeptidase from Mycobacterium tuberculosis: domain B and C== | | ==Crystal structure of LdtMt2, a L,D-transpeptidase from Mycobacterium tuberculosis: domain B and C== |
| - | <StructureSection load='4huc' size='340' side='right' caption='[[4huc]], [[Resolution|resolution]] 1.86Å' scene=''> | + | <StructureSection load='4huc' size='340' side='right'caption='[[4huc]], [[Resolution|resolution]] 1.86Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4huc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HUC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HUC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4huc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HUC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HUC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hu2|4hu2]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4huc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4huc OCA], [https://pdbe.org/4huc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4huc RCSB], [https://www.ebi.ac.uk/pdbsum/4huc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4huc ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lppS, MT2594, Rv2518c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4huc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4huc OCA], [http://pdbe.org/4huc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4huc RCSB], [http://www.ebi.ac.uk/pdbsum/4huc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4huc ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/LDT2_MYCTO LDT2_MYCTO] Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala(4) bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap(3) of the donor stem and the side chain of mDap(3) of the acceptor stem. Is specific for donor substrates containing a stem tetrapeptide since it cannot use pentapeptide stems. Is essential for virulence in a mouse model of acute infection.<ref>PMID:20305661</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Both, D]] | + | [[Category: Large Structures]] |
| - | [[Category: Lindqvist, Y]] | + | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: Schneider, G]] | + | [[Category: Both D]] |
| - | [[Category: Schnell, R]] | + | [[Category: Lindqvist Y]] |
| - | [[Category: Steiner, E]] | + | [[Category: Schneider G]] |
| - | [[Category: Extracellular]] | + | [[Category: Schnell R]] |
| - | [[Category: Peptidoglycan remodeling]] | + | [[Category: Steiner E]] |
| - | [[Category: Periplasmic]]
| + | |
| - | [[Category: Transpeptidase]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
LDT2_MYCTO Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala(4) bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap(3) of the donor stem and the side chain of mDap(3) of the acceptor stem. Is specific for donor substrates containing a stem tetrapeptide since it cannot use pentapeptide stems. Is essential for virulence in a mouse model of acute infection.[1]
Publication Abstract from PubMed
The transpeptidase LtdMt2 catalyzes the formation of the (3-3) cross-links characteristic of the peptidoglycan layer in the Mycobacterium tuberculosis cell wall. Bioinformatics analysis suggests that the extramembrane part of the enzyme consists of three domains: two smaller domains (denoted as A and B domains) and a transpeptidase domain (the C domain) at the C-terminus. The crystal structures of two fragments comprising the AB domains and the BC domains have been determined. The structure of the BC module, which was determined to 1.86 A resolution using Se-SAD phasing, consists of the B domain with an immunoglobulin-related fold and the catalytic domain belonging to the ErfK/YbiS/YbnG fold family. The structure of the AB-domain fragment, which was solved by molecular replacement to 1.45 A resolution, reveals that despite a lack of overall sequence identity the A domain is structurally very similar to the B domain. Combining the structures of the two fragments provides a view of the complete three-domain extramembrane part of LdtMt2 and shows that the protein extends at least 80-100 A from the plasma membrane into the peptidoglycan layer and thus defines the maximal distance at which cross-links are formed by this enzyme. The LdtMt-related transpeptidases contain one or two immunoglobulin domains, which suggests that these might serve as extender units to position the catalytic domain at an appropriate distance from the membrane in the peptidoglycan layer.
Structure of LdtMt2, an L,D-transpeptidase from Mycobacterium tuberculosis.,Both D, Steiner EM, Stadler D, Lindqvist Y, Schnell R, Schneider G Acta Crystallogr D Biol Crystallogr. 2013 Mar;69(Pt 3):432-41. doi:, 10.1107/S0907444912049268. Epub 2013 Feb 16. PMID:23519418[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gupta R, Lavollay M, Mainardi JL, Arthur M, Bishai WR, Lamichhane G. The Mycobacterium tuberculosis protein LdtMt2 is a nonclassical transpeptidase required for virulence and resistance to amoxicillin. Nat Med. 2010 Apr;16(4):466-9. doi: 10.1038/nm.2120. Epub 2010 Mar 21. PMID:20305661 doi:http://dx.doi.org/10.1038/nm.2120
- ↑ Both D, Steiner EM, Stadler D, Lindqvist Y, Schnell R, Schneider G. Structure of LdtMt2, an L,D-transpeptidase from Mycobacterium tuberculosis. Acta Crystallogr D Biol Crystallogr. 2013 Mar;69(Pt 3):432-41. doi:, 10.1107/S0907444912049268. Epub 2013 Feb 16. PMID:23519418 doi:10.1107/S0907444912049268
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